Effects of β-TCP Ceramics on Osteoblast Cellular Proliferating,Mineralization and Osteocalcin Expression  

作　　者：QI Zhitao ZHANG Qihuan ZHENG Qiang DAI Honglian WANG Zisheng QIU Ming LI Shipu 齐志涛;张启焕;ZHENG Qiang;DAI Honglian;WANG Zisheng;QIU Ming;LI Shipu(School of Chemical and Biological Engineering,Yancheng Institute of Technology;The Bio-material Research and Engineering Center,Wuhan University of Technology;Yancheng Tianbang Feed Technology Co.Ltd,Yancheng 224056,China)

机构地区：[1]School of Chemical and Biological Engineering,Yancheng Institute of Technology [2]The Bio-material Research and Engineering Center,Wuhan University of Technology [3]Yancheng Tianbang Feed Technology Co.Ltd,

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2012年第1期107-109,共3页武汉理工大学学报（材料科学英文版）

基　　金：the Research Fund of Key Labortary for Advanced Technology in Environmental Protection of Jiangsu Province (No. AE201037);the Foundation for Talent Recruitment of Yancheng Institute of Technology (No. XKR2011007);the "973" Chinese National Key Fundamental Research and Development Program (No. G1999064701)

摘　　要：After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.

关 键 词：β-TCP ceramics OSTEOBLAST cellular proliferating MINERALIZATION OSTEOCALCIN 

分 类 号：R687.3[医药卫生—骨科学] R318.1[医药卫生—外科学]