枯草芽孢杆菌淀粉酶高产菌株的复合诱变研究  

Compound mutation of Bacillus subtilis with high-yield of amylase

作　　者：马颖辉[1] 王景会[2] 李玉秋[2] 高岩[3] 曹龙奎[1] 

机构地区：[1]黑龙江八一农垦大学,黑龙江大庆163319 [2]吉林省农业科学院,吉林长春130124 [3]吉林农业大学,吉林长春130175

出　　处：《中国酿造》2012年第2期53-56,共4页China Brewing

基　　金：吉林省科学技术厅资助项目(20090248)

摘　　要：该研究以实验室保存的枯草芽孢杆菌为出发菌株,利用紫外和硫酸二乙酯(DES)复合诱变的方法,从大量突变菌株中获取可高效发酵生产淀粉酶的菌株。又通过连续传代实验,确定了该突变菌株突变性状能稳定遗传。结果表明,菌株D-2具有较高的的淀粉酶活力,其淀粉酶活力可达到6.599U/mL,较出发菌株提高到4倍。并在连续培养5代后,仍具有较好的遗传稳定性。该文探讨了一种高效的发酵生产淀粉酶菌株的选育方法,为下一步菌种生长条件优化打下了基础。The Bacillus strains preserved in our laboratory was used as the original strain to be mutated using UV mutagenesis and diethyl sulfate in order to increase the production of amylase. A mutant strain which was stable in hereditary was obtained. The results indicated that the strain of D-2 had a high production of amylase. The yield of amylase reached 6.599U/ml, which was 4 folds higher than that of the starting strain. A new efficient starch-hydrolysis enzyme strain screening method was developed, which made a good foundation for further medium optimization.

关 键 词：复合诱变 枯草芽孢杆菌 淀粉酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]