侗家奇液酒中游离氨基酸含量测定分析  

Determination and analysis of free amino acids content in Dongjiaqiye wine

作　　者：王翔[1,2] 杨晓燕[1] 张钰萍[2] 

机构地区：[1]凯里学院应用化学研究所,贵州凯里556011 [2]贵州大学精细化工研究开发中心与教育部绿色农药和农业生物工程重点实验室,贵州贵阳550025

出　　处：《中国酿造》2012年第2期182-186,共5页China Brewing

基　　金：凯里学院服务地方经济社会发展专项项目(ZX1037);贵州省教育厅自然科学基金青年项目(黔教科20090079);贵州省特色重点学科项目(黔教高发【2011】208号)资助

摘　　要：采用日立L-8800型氨基酸自动分析仪分析研究侗家奇液酒中游离氨基酸的组成，色谱条件：标准分析柱4．6mm×60．Omm，反应柱温度：150℃，测定了不同地区保健酒中游离氨基酸的含量。结果表明，所测试样中各种氨基酸加标回收率均在85．2％-112．6％，最低检出限为3pmol。该仪器重现性好，实验数据可靠。测定的侗家奇液酒中游离氨基酸总含量为488．48mg／L，仅次于灵芝酒；谷氨酸含量为252．12mg／L，占氨基酸总量的51．59％，在6种保健酒中含量最高，是一种天然绿色、营养健康的保健佳品。The contents of free amino acids in the Dongjiaqiye wine from different region were determined by the Hitachi L-8800 amino acid mobile analyzer. The chromatographic condition were as followed： standard column 4.6mm×60.0mm and temperature of reactive column 150℃. The results showed that the recoveries of all samples were in the range 85.2%-112.6% and the lowest detection limit was 3pmol. It was indicated that the instru- ment reproduction was good and the experimental data was reliable. The totals free amino acids in Dongjiaqiye wine was 488.48mg/L, which was only lower than that of Ganoderma wine. The glutamic acid content was 252.12mg/L which accounted for 51.59% of total amino acids, and its con- tent was highest in six kinds of health wine. It is a natural and nutritional food.

关 键 词：L-8800型氨基酸分析仪 侗家奇液酒 游离氨基酸 

分 类 号：O657.7[理学—分析化学]