3种疾病肾阳虚证“同证异治”的信号通路调控研究  

Research to Three Kinds of Disease Kidney Yang Syndrome"Treating Same Pattern with Different Method"at Signal Transduction Pathways

作　　者：严石林[1] 沈宏春[1,2] 王浩中[1] 鲁法庭[1] 于宏波[3] 陈为[1] 陶怡[1] 

机构地区：[1]成都中医药大学,四川成都610075 [2]泸州医学院,四川泸州646000 [3]云南中医学院,云南昆明650500

出　　处：《云南中医学院学报》2012年第1期5-9,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然基金(NO:30873237)

摘　　要：目的:研究患慢性肾炎、糖尿病肾病、阳痿病中筛选出的典型肾阳虚证的转录组学特征,揭示肾阳虚证"同证异治"的生物学基础。方法:分别对9例不同疾病的肾阳虚证,进行Agilent人4*44k表达谱芯片实验,对差异表达基因进行Pathway分析。结果:找出3种不同疾病肾阳虚证的共同差异表达基因332条,其中有注释的基因为181条。3种不同疾病肾阳虚证共同的信号通路有37个。并从3组疾病各自特有的差异表达基因中发现mTOR、notch、Wnt等3条信号通路与肾阳虚证共有的信号通路一致,只是在通路的调控上有基因表达差异。结论:3种疾病相关的特有差异表达基因中的3条信号通路差异调控,可能决定了肾阳虚证"同证异治"的转录组学基础。Objective： to study with chronic nephritis, diabetic nephropathy, impotence disease were studied in typical kidney Yang syndrome transcription group learning characteristics, this paper reveals the kidney Yang syndrome ＂treating same pattern with different method＂ the biological basis. Methods： to 9 cases were dif- ferent disease of kidney Yang syndrome, Agilent people 4. 44k expression profile chip experiments, the difference between the gene expression Pathway analysis. Results： find out three different disease kidney Yang syndrome of common article 332 differentially expressed genes, including the comment for article 181 genes. Three different disease kidney Yang syndrome common signal pathway is 37. And from the three groups of disease their unique differentially expressed genes found in mTOR, notch, Wnt as 3 signal path and kidney Yang syndrome were signal path consistent, just in the path of the regulation on the gene expression differences. Conclusion： three diseases of the special differentially expressed genes related to signal transduction article three of the differences may decide the kidney Yang syndrome ＂treating same pattern with different method＂ the basic of transcriptomics.

关 键 词：肾阳虚证 转录组学 同证异治 

分 类 号：R228[医药卫生—中医基础理论]