共轭亚油酸对小鼠乳腺脂肪合成相关基因表达的作用研究  

EFFECT OF CONJUGATED LINOLEIC ACID ON THE EXPRESSION OF LIPOGENIC GENES IN MOUSE MAMMARY GLAND

作　　者：韩立强[1] 郭豫杰[1] 鲁维飞[1] 朱河水[1] 杨国庆[1] 杨国宇 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]农业部动物生化与营养重点实验室,郑州450002

出　　处：《营养学报》2012年第1期68-73,共6页Acta Nutrimenta Sinica

基　　金：国家973项目子课题(No.2011CB100802)

摘　　要：目的研究共轭亚油酸(CLA)对小鼠乳腺脂肪合成相关基因表达的影响。方法选取32只泌乳昆明鼠,随机分为对照组(2%花生油);0.5%CLA组(0.5%CLA+1.5%花生油);1.0%CLA组(1%CLA+1%花生油);1.5%CLA组(1.5%CLA+0.5%花生油),每组8只小鼠,从泌乳第4日饲喂至第14日,每日检测母鼠采食量、体重和仔鼠窝重,分析乳成分,采用荧光定量PCR检测对照组和1.5%CLA组母鼠乳腺组织脂肪合成相关基因的表达。结果在饲喂期间各组母鼠体重没有差异,饲料添加1.5%CLA显著减少了母鼠的采食量、仔鼠窝重和乳脂肪含量(P<0.05)。与对照组相比,1.5%CLA组母鼠乳腺组织的脂蛋白脂酶(LPL)、乙酰辅酶A羧化酶(ACACA)、硬脂酰辅酶A去饱和酶(SCD)和脂肪酸合成酶(FASN)的基因表达显著降低(P<0.05),并且转录因子固醇调节元件结合蛋白(SREBF)和过氧化物酶体增殖激活受体(PPARγ)的基因表达也显著下调(P<0.01)。结论饲料中加入1.5%CLA能够减少泌乳母鼠乳脂肪含量,抑制乳腺脂肪合成相关基因的表达。Objective To investigate the effect of conjugated linoleic acid（CLA） on the expression of lipogenic gene in mammary gland of mice.Method The lactating KM mice were randomly assigned to four groups（n=8 for each group）.control group（2% peanut oil）,0.5% CLA group（0.5% CLA ＋1.5% peanut oil）,1.0% CLA group（1%CLA＋1% peanut oil） and 1.5% CLA group（1.5%CLA＋0.5% peanut oil）.All animals were fed the diet from D 4 to D 14 postpartum.The food intake,body weight of dams and litters were assessed.The content of milk fat,protein and lactose was determined.The expression of lipogenic genes was analyzed in mammary tissue between control and 1.5% CLA group via Real-time PCR.Results The body weight of dams was unaffected by treatment.The 1.5%CLA diet siginificantly decreased the food intake of dams,body weight of litters and milk fat of dams（P0.05）.The expression of lipoprotein lipase（LPL）,acetyl-CoA carboxylase（ACACA）,stearoyl-CoA desaturase（SCD） and fatty acid synthase（FASN） genes were significantly inhibited in mammary tissue by the intake of 1.5%CLA（P0.05）.The expression of transcription factor（sterol regulatory element binding factor,SREBF and peroxisome proliferators-activated receptor γ,PPARγ） genes were also down-regulated in mammary tissue in 1.5%CLA group compared with control（P0.01）.Conclusion Supplementing the diet with 1.5%CLA significant decreased the content of milk fat and inhibited the expression of lipogenic genes in mammary gland of lactating mouse.

关 键 词：共轭亚油酸 泌乳 乳脂肪 基因表达 

分 类 号：S816[农业科学—饲料科学] S865.13[农业科学—畜牧学]