成人急性白血病GST-π与LRP基因的RQ-PCR检测及其临床意义  

RQ-PCR Detection of GST-π and LRP Genes in Adult Acute Leukemia and Its Clinical Significance

作　　者：王静[1] 肖镇[1] 

机构地区：[1]内蒙古医学院附属医院血液科,内蒙古自治区呼和浩特010059

出　　处：《中国实验血液学杂志》2012年第1期78-82,共5页Journal of Experimental Hematology

基　　金：内蒙古自然科学基金;编号200711020952

摘　　要：本研究通过对急性白血病(AL)患者外周血谷胱甘肽-S-转移酶-π(GST-π)和肺耐药相关蛋白(lungresistance-related protein,LRP)基因的检测,探讨两种基因与患者多药耐药性的关系。采用实时荧光定量逆转录-聚合酶链反应(RQ-PCR)检测44例AL患者及27例正常人外周血单个核细胞GST-π与LRP基因的表达。结果表明,GST-π基因水平在初治组和难治组与完全缓解组之间均有极显著性差异(P<0.01)。LPR基因水平在初治组与难治组,完全缓解组与难治组均有极显著性差异(P≤0.01)。GST-π与LRP基因之间无相关性。GST-π及LRP基因表达在不同外周血白细胞计数组、不同临床分型组(ALL、ANLL)之间的差异均不显著。结论:GST-π和LRP基因通过不同机制引起多药耐药,二者联合检测较单独测定某一基因对白血病评定预后更具临床意义。外周血白细胞计数、白血病分型可能与GST-π和LRP基因的表达无关。This study was aimed to detect the glutathion S-transferase-qr （GST-π） and lung resistance-related protein （LRP） genes and to investigate their relationship with multidrug resistance（MDR） of patients with acute leukemia（AL）. Real-time fluorescent quantitative reverse transcription polymerase chain reaction （RQ-PCR） was used to detect the expression of GST-π and LRP genes in peripheral blood mononuclear cells from 44 AL patients and 27 normal subjects. The results showed that the significant difference in GST-~ expression level was found between newly diagnosed patients and complete remission patients and between refractory patients and complete remission patients （ P 〈 0. 01 ）, while expression level of LRP genes showed obvious difference （ P〈 0.01 ） between newly diagnosed patients and refractory patients and between complete remission patients and refractory patients. Statistical analysis indicated that there was no correlation between GST-π gene and LRP gene. The expression of GST-~ and LRP genes was not significantly different in different white blood cell（WBC） count groups and different clinical typing groups （ALL and ANLL）. It is concluded that the mechanism of MDR resulting from GST-π and LRP genes is different, thereby combination detection of GST-π and LRP genes demonstrates a larger role for evaluating prognosis of AL patients, as compared with detetion of GST-,rr or LRP gene alone. The WBC count and leukemia typing have no relationship with expression of GST-,rr and LRP genes.

关 键 词：GST-Π LRP MDR 白血病 RQ-PCR 

分 类 号：R733.71[医药卫生—肿瘤]