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作 者:谢佳[1,2] 张梅[1] 宋艳萍[1,2] 胡凯[2] 任婧婧[2] 张韵洁[2]
机构地区:[1]西安交通大学医学院第一附属医院血液病研究室,陕西西安710061 [2]西安市中心医院血液病研究所,陕西西安710003
出 处:《中国实验血液学杂志》2012年第1期107-111,共5页Journal of Experimental Hematology
摘 要:本研究探讨三氧化二砷(As2O3)作用于骨髓瘤细胞RPMI8226引起非Caspase依赖性细胞凋亡。用MTT法检测细胞增殖率,流式细胞术检测RPMI8226细胞的凋亡率,Western blot方法检测细胞BCL-2及Caspase-3蛋白表达水平。结果表明,As2O3(0.1-20μmol/L)作用于人骨髓瘤细胞RPMI8226 24,48,72 h显示出明显的增殖抑制作用(P<0.05),呈浓度和时间依赖性。与As2O3(10μmol/L)单独处理组相比,zVAD-fmk(20μmol/L)与As2O3联合处理组的凋亡率未见明显变化。与As2O3(10μmol/L)单独处理组比较,zVAD-fmk与As2O3联合处理组Caspase-3、BCL-2蛋白表达明显升高。结论:As2O3对RPMI8226细胞的增殖有明显的抑制作用。As2O3可诱导RPMI8226细胞发生凋亡,其凋亡过程中可能存在Caspase非依赖性细胞凋亡程序。This study was purposed to explore the caspase-independent apoptosis pathway in human multiple myelo- ma cell RPMI8226 induced by arsenic trioxide ( As2 03 ). MTT method was used to analyze the proliferation inhibition rate; flow cytometry was used to detect the apoptosis rate; Western blot was used to determine the expressions of BCL-2 and Caspase-3 in RPMI8226 cells. The results showed that As203 (0.1 -20 ixmoVL) significantly inhibited the prolifer- ation of RPMI8226 ( P 〈 0.05 ) in concentration- and time-dependent manner. Compared with the group treated with As203 (10 txmol/L) alone, the apoptosis rate of zVAD-fmk(20 ixmol/L) and As203 combined treated group did not change. Compared with the group treated with As203 (10 pjnol/L) alone, zVAD-fmk(20 ~tmol/L) combined with As203 ( 10 pjnol/L) treatment group showed significant increase of expressions of Caspase-3 and BCL-2. It is concluded that As203 can inhibit the proliferation of RPMI8226 cells. As203 can induce apoptosis of RPMI8226 ceils, and a caspase-independent process probably exist in As2 03-inducing RPMI8266 cells apoptosis.
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