降胆固醇干酪乳杆菌HC12的培养条件优化  

Optimization of Culture Conditions of Cholesterol-lowering Lactobacillus casei HC12

作　　者：刘长建[1] 姜波[1] 蒋本国[1] 闫建芳[1] 刘秋[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600

出　　处：《中央民族大学学报（自然科学版）》2012年第1期18-21,27,共5页Journal of Minzu University of China(Natural Sciences Edition)

基　　金：国家自然科学基金(No.30671398);NSFC-KOSEF国际合作项目(No.30711140389)

摘　　要：干酪乳杆菌HC12具有降低培养基中胆固醇的能力,通过单因子、正交试验对其培养条件和培养基进行了优化.在MRS培养基的基础上,培养基最佳配比为酵母粉2.0%,葡萄糖2.5%,胰蛋白胨1.5%,牛肉膏1.0%;最适的起始pH值为6.0,最适温度为28℃.优化后的培养基、培养条件使干酪乳杆菌HC12的生长量(OD值)达到了1.502,去除胆固醇的量达到54.3 ug/mL.Culture medium and conditions of Lactobacillus casei HC12 were optimized by single-factor and orthogonal experiments.Baced on MRS medium,several compoments of MRS medium were optimized as follows： yeast extract powder 2%,glucose 2.5%,tryptone 1.5%,beef extract 1.0%.Its optimal culture conditions were as follows ： initial pH 6.0;temperature of fermentation 28 ℃.The growth（OD value） of strain HC12 was up to 1.502,and the ability of cholesterol removal reached 54.3 ug/mL in the optimum culture medium and conditions.

关 键 词：干酪乳杆菌 培养条件 OD值 胆固醇去除能力 

分 类 号：Q939.9[生物学—微生物学]