恶性肿瘤电化学治疗前后细胞周期分析及病理学改变  

Cell Cycle Analysis and Pathological Changes of Malignant Tumors Treated with Electrochemical Therapy

作　　者：刘继彦[1] 魏于全[1] 罗锋[1] 卢实春[1] 彭玉兰[1] 雷松[1] 赵霞[2] 严永华[3] 

机构地区：[1]华西医科大学附属第一医院,成都610041 [2]华西医科大学附属第二医院妇产科,成都610041 [3]四川省劳动卫生职业病防治研究所

出　　处：《华西医科大学学报》2000年第1期104-106,共3页Journal of West China University of Medical Sciences

摘　　要：为探讨电化学疗法对肿瘤细胞的作用及治癌机理 ,采用流式细胞术及病理学方法 ,观察了 33例晚期癌症患者电化学治疗前后肿瘤细胞周期及病理学变化。结果 :电化学治疗后肿瘤各期细胞所占比例均明显降低 ,而细胞凋亡峰却明显增加 ,治疗后 G1 、G2 / M、S期的降低及凋亡峰的增加有显著的统计学差异 (P<0 .0 5 ) ;病理学结果亦支持电化学疗法对肿瘤细胞的杀伤作用。以上提示 ,电化学疗法能非选择性地杀伤包括肝癌、淋巴瘤、乳腺癌及皮肤癌在内的各种肿瘤细胞 ,能非选择性地杀伤各期肿瘤细胞 ,并诱导肿瘤细胞的凋亡 ;流式细胞周期分析可作为电化学疗法有效的判断指标。To investigate the mechanism and to observe the effectiveness of electrochemical therapy(ECT),33 patients of late stage cancers which treated by ECT were included in this study.Flow cytometry(FCM)and pathology were used to observe the changes of tumor cells before and after ECT.Tumor cells of G 1,G 2/M,S phases and aneuploid cells were found to be killed almostly and the ratio of apoptosis was elevated greatly after ECT.Also, pathological evidence proved the death of tumor cells and some characters of apoptosis.So, ECT can non selectively kill tumor cells and induce the apoptosis of them, and FCM was a way to evaluate the effectiveness of ECT.

关 键 词：恶性肿瘤 电化学疗法 细胞凋亡 病理学 

分 类 号：R730.2[医药卫生—肿瘤]