全明星草莓乙烯受体Ers1基因克隆及序列分析  

Cloning and Sequence Analysis of a DNA Encoding Ethylene ReportorErs1 from Starwberry

作　　者：刘思思[1] 马俊莲[1] 唐霞[1] 张子德[1] 宋春丽[2] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]河北农业大学中兽医学院,河北定州073000

出　　处：《华北农学报》2011年第5期46-49,共4页Acta Agriculturae Boreali-Sinica

基　　金：河北省科技攻关项目(04395501D-3)

摘　　要：克隆了与草莓成熟有关的乙烯受体Ers1基因片段,为进一步研究Ers1基因功能并通过基因技术改善草莓贮运性能奠定基础。以全明星草莓成熟果实中分离得到基因组DNA为模板,经PCR扩增到一条约700 bp的特异片段,将该片段克隆到PEGM-T easy vector上经测序分析,基因全长667 bp,编号222个氨基酸。序列分析结果表明,该序列与Chandler-Ers1的cDNA序列同源性99%,氨基酸序列同源性98%。In this study a ripening related ethvlene recepor Ersl gene in strawberry fruits was cloned for the following-up reseach on gene and improving varieties of strawberry through transgenic technology. A pair of primers were designed and synthesized based on the sequence reported of Chandler-Ersl. Using genome DNA extracted from All-star strawberry mature fruits as template the specific PCR product of Ersl was obtained, then it was ligated to pEGM-T easy vector and sequenced. The sequencing data showed that the PCR product was 667 bp encoding 222 peedicted amino acid residues. Comparison with the eDNA sequence from Chandler-Ersl indicated the homlogy was 98% ,and amino acid identitise were 97%.

关 键 词：草莓 乙烯受体Ers1 基因克隆 序列分析 

分 类 号：S668.4[农业科学—果树学] Q78[农业科学—园艺学]