茶多酚对皮肤角朊细胞生长与凋亡的影响  

Effects of tea polyphenols on cell kinetics and apoptosis of primary cultured rat skin keratinocytes

作　　者：符移才[1] 魏少敏[2] 林惠芬 张英[3] 金锡鹏[1] 

机构地区：[1]上海医科大学预防医学研究所皮肤生理毒理研究室,上海200032 [2]上海家化有限公司科研部 [3]浙江大学食品科技系

出　　处：《卫生研究》2000年第2期94-96,共3页Journal of Hygiene Research

摘　　要：为探讨茶叶中的重要活性成分茶多酚对皮肤角朊细胞的生长周期动力学和对细胞凋亡的影响,在原代培养大鼠皮肤角朊细胞基础上,根据培养基中乳酸脱氢酶释放情况确定的茶多酚浓度范围,采用细胞计数法和流式细胞仪研究角朊细胞的生长、周期动力学和凋亡发生率。结果发现,在一定浓度范围内(0.1～1.0g/L),茶多酚可以促进角朊细胞生长。在细胞周期动力学上,表现为促进细胞从G1期和静止期(G0)转向有丝分裂合成期S和G2/M期,增殖指数(PI)也从18.17%增至25.62%;同时细胞凋亡发生率从27.10%下降到17.97%。表明茶多酚可以促进皮肤角朊细胞有丝分裂和生长,减少细胞凋亡发生。In order to study the effects of tea polyphenols on cell growth and apoptosis of primary cultured rat skin keratinocytes, the safe dosage of tea polyphenols was determined by the release of lactate dehydrogenase(LDH). Cell counting(cytometry) and flow cytometry(FCM) were used to estimate the cell growth and the percentages of cells in different cell cycle phases or apoptosis. The results showed that tea polyphenols stimulated the cell growth from G 1/G 0 to G 2/M phase, enhanced the synthesis of DNA(increase S phase) and increased the proliferative index(PI) from 18 17% to 25 62%. Also, it could block the occurrence of apoptosis. It is concluded that tea polyphenols could stimulate the growth of skin keratinocytes through stimulating the synthesis of cell DNA and inhibiting apoptosis.

关 键 词：茶多酚 皮肤角朊细胞 细胞生长 细胞凋亡 肿瘤 

分 类 号：R730.1[医药卫生—肿瘤]