微囊化人胃癌细胞SGC-7901模型建立  

Establishment of the cultured model of microencapsulated human gastric carcinoma cells SGC-7901

作　　者：冀学宁[1] 范学军[1] 全秀莲[1] 王若雨[1] 王为[2] 马小军[2] 

机构地区：[1]大连大学附属中山医院肿瘤科,116001 [2]大连中科院化学物理研究所

出　　处：《中华实验外科杂志》2012年第3期543-545,共3页Chinese Journal of Experimental Surgery

基　　金：大连市卫生局科技项目

摘　　要：目的建立微囊化人胃癌细胞株SGC-7901培养模型。方法微胶囊制备仪包裹SGC-7901细胞，培养至21d，噻唑蓝（MTr）比色法观察隔日细胞的生长活性，生物传感分析仪检测隔日培养上清中葡萄糖和乳酸浓度；选取第7、14、21天的微囊化SGC-7901细胞，苏木素-伊红（HE）染色；免疫细胞化学法分别观察微囊前后SGC-7901细胞5一溴脱氧尿嘧啶核苷（5-BrdU）、细胞核抗原（PCNA）及血管内皮生长因子（VEGF）基因表达。结果MTT结果示培养至第14天，细胞相对数不再增加，随后细胞相对数略下降；微囊化人胃癌细胞培养上清中葡萄糖含量逐渐降低，乳酸上升；至20d后分别为10、100mmol／L。免疫细胞化学法检测SGC-7901细胞贴壁培养中表达PCNA和VEGF基因；第7和14天，微囊化人胃癌细胞可见PCNA、BrdU及VEGF基因表达；21d，PCNA和Br-dU主要在细胞团外层表达，内层细胞则表达少或不表达，而VEGF均见表达。结论呈三维立体生长的微囊化胃癌细胞模型生长稳定，相关基因表达稳定。Objective To＇establish the cultured model of microencapsulated human gastric carci- noma （HGC） cell line SGC-7901. Methods The SGC-7901 cells were cultured and encapsulated in APA microcapsules using the prepared electrostatic droplet generator for 21 days. The level of proliferation and metabolism of the microencapsulated HGC cells were detected by using methyl thiazol tetrazolium （ MrlT）, and glucose and lactic acid concentrations were determined in the supernatant. Microencapsulated HGC cells were stained with hematoxylin and eosin （ HE）, and the expression of bromodeoxyuridine （ BrdU）, vascular endothelial growth factor （VEGF） and proliferating cell nuclear antigen （PCNA） gene was detec- ted by using immunocytochemistry on the 7th, 14th and 21st day. Monolayer cultured cells served as con- trois. Results On the 14th day, the relative number of the microencapsulated cells was no longer in- creased and decreased slightly on the next few days. Glucose was consumed and its concentration was de- creased to 10 mmol/L, and lactic acid was generated and its concentration was increased to 100 mol/L in mieroencapsulated HGC cells on the 20th day. The expression of PCNA and VEGF gene could be detected in SGC7901 cells. The expression of BrdU, PCNA and VEGF was still detected in the microencapsulated SGC9701 cells on the 7th and 14th day. On the 21st day, the expression of Brdu and PCNA was found on the outer layer of spheroid, and that of VEGF gene was detected within the whole spheroid. Conclusion The cultured model of microencapsulated HGC SGC9701 had been established, and gene expression was stable.

关 键 词：胃癌 微囊化 溴脱氧尿嘧啶核苷 细胞核抗原 血管内皮生长因子 

分 类 号：R735[医药卫生—肿瘤]