HPLC柱后衍生同时测定黄曲霉毒素B_1、B_2、G_1、G_2的探讨  

Simultaneous determination of aflatoxins B_1,B_2,G_1,G_2 by HPLC with postcolumn derivatization

作　　者：王新丽[1] 张玉黔[2] 

机构地区：[1]沈阳市铁西区疾病预防控制中心,沈阳110021 [2]沈阳市疾病预防控制中心,沈阳110031

出　　处：《中国卫生检验杂志》2012年第2期225-226,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立黄曲霉毒素B1、B2、G1、G2的高效液相色谱柱后衍生检测方法。方法:样品通过高效液相色谱柱分离后,进入柱后衍生装置与碘溶液发生反应,最后进入荧光检测器进行检测。结果:该方法的检出限AFB1、AFG2为0.03μg/kg、AFB2为0.01μg/kg、AFG1为0.05μg/kg。结论:方法灵敏度高,准确度好,操作简单,实用性强,可用于黄曲霉毒素的检测。Objective：To develop a high performance liquid chromatography with postcolumn derivatization method for simulta- neous determination of Aflatoxins B1 , B2, G1 , G2. Methods： The extracted samples were separated by HPLC column, conducted reaction with iodine solution in postcolumn derivatization device, finally detected by fluorescence detector. Results： The method has a limit of detection of 0.03 μg/kg for aflatoxins B1, G2, 0.01 μg/kg for aflatoxin B2 and 0.05 μg/kg for aflatoxin G1. Con- cluslon： This method was sensitive, accurate, simple and specific, which can be applied for the determination of aflatoxins B1, B2, Gl, G2.

关 键 词：高效液相色谱法 柱后衍生 黄曲霉毒素 

分 类 号：R735.7[医药卫生—肿瘤]