Parthenolide attenuates LPS-induced activation of NF-κB in a time-dependent manner in rat myocardium  

作　　者：Hong Xie Chen Wang Xuemei Wu Xia Liu Shigang Qiao Chunfeng Liu Hong Liu 

机构地区：[1]Department of Anesthesiology, the Second Affiliated Hospital of Sooehow University, Suzhou 215004, China [2]Institute of Neuroseienee, Sooehow University, Suzhou 215004, China [3]Department of Anesthesiology and Pain Medicine, University of California Davis, Davis, CA 95616, USA

出　　处：《The Journal of Biomedical Research》2012年第1期37-43,共7页生物医学研究杂志（英文版）

基　　金：supported by grant No.30872453 (to Dr.Wang) from National Natural Science Foundation of China;No.BK2008166 (to Dr.Wang) from Natural Science Foundation of Jiangsu Province;No.2008-11 (to Dr.Wang) from Technology Bureau of Suzhou,China;No.08KJD320005 (to Dr.Xie) from Natural Science Research Projects of Colleges and Universities in Jiangsu Province,China

摘　　要：Parthenolide （PTN）, a selective nuclear factor kappa B （NF-κB） inhibitor, has been used extensively to inhibit NF-κB activation. The duration of the inhibitory effect of PTN on NF-kB in vivo remains unclear. This study was to determine whether a lipopolysaccharide （LPS） challenge 6, 12 and 24 h after the administration of PTN could activate NF-κB. Rats were devided into five groups. The rats in the PTN, PTN＋LPS and DMSO groups were injected intraperitoneally with PTN or DMSO. After 6, 12 or 24 h, LPS was administered in LPS and PTN＋LPS groups. The expressions of NF-κB p50, IκBα and p-IκBα were inhibited in both PTN and PTN＋LPS group at end of 6 and 12 h and no effects at 24 h. In summary, myocardial NF-κB expression occurs 1 h after the administration of LPS. PTN blocks this effect given at 6 h and no inhibitory effect 24 h after administration in vivo.Parthenolide （PTN）, a selective nuclear factor kappa B （NF-κB） inhibitor, has been used extensively to inhibit NF-κB activation. The duration of the inhibitory effect of PTN on NF-kB in vivo remains unclear. This study was to determine whether a lipopolysaccharide （LPS） challenge 6, 12 and 24 h after the administration of PTN could activate NF-κB. Rats were devided into five groups. The rats in the PTN, PTN＋LPS and DMSO groups were injected intraperitoneally with PTN or DMSO. After 6, 12 or 24 h, LPS was administered in LPS and PTN＋LPS groups. The expressions of NF-κB p50, IκBα and p-IκBα were inhibited in both PTN and PTN＋LPS group at end of 6 and 12 h and no effects at 24 h. In summary, myocardial NF-κB expression occurs 1 h after the administration of LPS. PTN blocks this effect given at 6 h and no inhibitory effect 24 h after administration in vivo.

关 键 词：PARTHENOLIDE nuclear-factor-κB LIPOPOLYSACCHARIDE MYOCARDIUM 

分 类 号：Q939.1[生物学—微生物学] S858.237.2[农业科学—临床兽医学]