鱼鳞胶原—壳聚糖止血海绵的制备工艺  

Research on preparation technique of fish scale collagen-chitosan hemostatic sponge

作　　者：王茵 黄煜[2] 许永安[1] 吴成业[1] 

机构地区：[1]福建省水产研究所,厦门361012 [2]福建农林大学食品科学学院,福州350002

出　　处：《渔业科学进展》2012年第1期129-135,共7页Progress in Fishery Sciences

基　　金：福建省自然科学基金(2008J0251);福建省水产研究所科研发展基金资助项目共同资助

摘　　要：以罗非鱼Tilapia mossambica鱼鳞为原料,经低温酶解提取鱼鳞Ⅰ型胶原,将鱼鳞Ⅰ型胶原与壳聚糖进行混合交联,冻干后制成胶原-壳聚糖止血海绵。以止血海绵的密度、吸水倍数、保水率、透气率和止血时间为指标,通过单因素实验和正交试验优化获得最佳的制备工艺:胶原与壳聚糖配比7∶3、制备液总浓度1.4%、交联剂添加量0.015%。在该最佳工艺下制备的止血海绵密度为22.95mg/cm3、吸水倍数为45.34、保水率为55.83%、透气率为46.36%、止血时间为68.0s,呈蜂窝状多孔结构,具有优良的持水性和透气性。Fish scale type I collagen was extracted through low temperature enzymolysis from TilaDia mossambica, and after being crosslinked with chitosan, hemostatic sponge wasgenerated via freeze-drying. Through single factor comparative tests and orthogonal tests, the optimum preparation technique was obtained as follows： mixing of collagen with chitosan according to a given volume ratio 7 ： 3, maintaining total concentration of preparation mixture at1.4%, and keeping dosage of cross-linking agent at 0. 015%. Under this condition, the generated hemostatic sponge possessed excellent properties, such as density （22.95 mg/cm^3）, waterabsorption （45.34）, water retention （55.83%）, breathability （46.36%）, and hemostatic time （68.0 s）. A faveolate vesicular structure, which contributed to preferable properties, was clearly observed with SEM.

关 键 词：鱼鳞胶原 壳聚糖 止血海绵 交联 

分 类 号：S986.1[农业科学—捕捞与储运]