荧光原位杂交在慢性粒细胞白血病细胞BCR/ABL融合基因检测中的应用及其意义  

The detection of BCR/ABL fusion gene by interphase fluorescence in situ hybridization in patients with chronic myeloid leukemia and its clinical significance

作　　者：张济[1] 李君君[1] 颜家运[1] 邹礼衡[1] 刘静[2] 

机构地区：[1]南华大学附属第一医院血液科,湖南省衡阳市421001 [2]中南大学生物科学与技术学院分子生物学研究中心,长沙市410078

出　　处：《实用医学杂志》2012年第5期825-827,共3页The Journal of Practical Medicine

基　　金：湖南省教育厅科研基金资助项目(编号:10C1161)

摘　　要：目的:探讨荧光原位杂交(FISH)技术检测慢性粒细胞白血病(CML)骨髓和外周血细胞的BCR/ABL融合基因的临床价值。方法:应用FISH对正常对照组及CML患者骨髓和外周血细胞BCR/ABL融合基因进行检测和分析。结果:慢性期CML患者骨髓和外周血细胞该融合基因阳性细胞率分别为(61.9±22.3)%和(68.4±19.8)%,加速期为(77.2±16.7)%和(86.8±12.1)%,急变期为(80.6±17.5)%和(81.4±18.0)%,两者细胞中BCR/ABL基因的阳性率未见统计学差异(P>0.05),且骨髓和外周血细胞之间融合基因阳性细胞比率呈直线正相关。同时发现在完全临床缓解患者中,经伊马替尼治疗的CML患者(71.4%)较经干扰素和羟基脲联合治疗者(10.0%)有更高的分子生物学缓解(P<0.05)。结论:通过FISH对CML患者骨髓和(或)外周血细胞融合基因进行监测,有助于CML的诊断、治疗及微小残留白血病的监测。Objective To explore the clinical significance of the detection of BCR/ABL fusion gene in bone marrow and peripheral blood cells by fluorescence in situ hybridization （FISH） in patients with chronic myeloid leukemia （CML）. Methods FISH technique was used to detecte BCR/ABL fusion gene in bone marrow and peripheral blood cells in healthy controls and CML patients. Results The positively expressing rates of BCR/ABL fusion gene in peripheral blood and bone marrow cells were （61.9 ± 22.3）% and （68.4 ± 19.8）% in patients with chronic phase of CML, were （77.2 ± 16.7）% and （86.8 ± 12.1）% in those with accelerating phase, and were （80.6 ± 17.5）% and （81.4 ± 18.0）% in those with blastic crisis, significant difference was not observed （P 0.05）. A significant correlation was found between the BCR/ABL positive cells in peripheral blood and in bone marrow samples. Moreover, the higher rate of complete molecular remission was achieved by treating with imatinib than with interferon and hydroxyurea during complete remission phase （P 0.05）. Conclusions Detecting BCR/ABL fusion gene in peripheral blood and bone marrow samples by FISH are beneficial to diagnosis, treatment and minimal residual disease monitor in CML.

关 键 词：白血病 髓系 慢性 BCR-ABL阳性 荧光原位杂交 BCR/ABL 融合基因 

分 类 号：R733.7[医药卫生—肿瘤]