黄山贡菊挥发油GC-MS指纹图谱研究  

GC-MS fingerprint analysis of volatile oil in Gong Chrysanthemum morifolium in Huangshan City

作　　者：高学玲[1] 贺曼曼[1] 岳鹏翔[1] 邹敏亮 查芳芳[1] 

机构地区：[1]安徽农业大学茶与食品科技学院,安徽合肥230036 [2]黄山天健农业科技有限公司,安徽黄山245700

出　　处：《食品工业科技》2012年第5期67-70,75,共5页Science and Technology of Food Industry

基　　金：国家星火计划项目(2010GA710027)

摘　　要：为建立黄山贡菊挥发油指纹图谱分析方法,采用GC-MS检测法并运用指纹图谱相似度评价软件对其结果进行分析。结果表明,采用HP-5MS毛细管色谱柱(0.25mm×30m×0.25μm),设置进样口温度为260℃,载气为高纯氦气,流速1.0mL.min-1,分流比为25:1,进样量2μL的条件,以程序升温对10批黄山贡菊挥发油成分进行分析,标定了黄山贡菊挥发油的59个共有峰为特征峰,初步建立了以59个共有峰为特征指纹信息的GC-MS指纹图谱,10批样品相似度均大于0.9;质谱鉴定出黄山贡菊挥发油含量最高成分为Bicyclo[3.1.1]hept-2-en-4-ol-2,6,6-trimethyl-aceate,约占总量的20%。此分析方法精密度高、稳定性及重现性好,挥发油中各成分分离效果较好,所建立的指纹图谱可为黄山贡菊质量控制提供参考,且上述含量最高的成分可作为黄山贡菊指标性成分之一来控制黄山贡菊质量的稳定性。To establish a method of fingerprint analysis on Gong Chrysanthemum morifolium in Huangshan City by using GC-MS and computer aided similarity evaluation.The results showed that the optimum condition of GC-MS was applied with a fiber column（0.25mm×30m×0.25μm） coated with HP-5MS.The temperature at feed inlet was 260℃,the carrier gas was nitrogen,the flow rate was 1.0mL·min-1,the split ratio was 25：1,and the sample size was 2μL.The essential oil in ten batches of Gongju was analyzed by adopting temperature programmer.In this condition,59 common peaks were identified and GC-MS fingerprint of 59 common peaks was established preliminarily.The correlation coefficients of ten batches of Gongju were higher than 0.9.The content of Bicyclo[3.1.1]hept-2-en-4-ol-2,6,6-trimethyl-aceate in volatile oil of Gongju was the highest（20%）.The method is of high degree of precision,reproducibility,and stability.The separating degree of each component in the essential oil is good.Both the established fingerprints and the component of Bicyclo[3.1.1]hept-2-en-4-ol-2,6,6-trimethyl-aceate could be used as the quality control index for Gong Chrysanthemum morifolium in Huangshan City.

关 键 词：黄山贡菊 挥发油 气-质联用仪 指纹图谱 

分 类 号：TS201.2[轻工技术与工程—食品科学]