高效液相色谱-Ru(bipy)_3^(2+)-Ce(SO_4)_2化学发光体系检测啤酒中的亚硫酸钠  

Determination of sodium sulfite in beer samples by high performance liquid chromatography with Ru(bipy)_3^(2+)-Ce(SO_4)_2 chemiluminescence detection

作　　者：龙星宇[1] 陈福南[1] 

机构地区：[1]西南大学化学与化工学院,重庆400715

出　　处：《食品工业科技》2012年第5期320-322,327,共4页Science and Technology of Food Industry

基　　金：西南大学博士基金项目(2120040511)

摘　　要：在酸性条件下,Ce(SO4)2氧化Ru(bipy)32+时,在Na2SO3存在下,对该化学发光具有很强的增敏作用,据此建立通过HPLC分离、用化学发光检测器测定亚硫酸钠的方法。在优化的实验条件下,测定亚硫酸钠的线性范围为2.0×10-6～5.0×10-4g/mL,方法的检出限为6.0×10-7g/mL,定量下限为2.0×10-6g/mL,线性回归方程:△I=3.578c+19.721(c:g/mL;r2=0.9984),对5.0×10-5g/mL亚硫酸钠进行了11次平行测定,其相对标准偏差为3.7%。该法已成功的运用于实际啤酒样中亚硫酸钠的含量。The CL radiation was emitted in the reaction of cerium sulfate oxidation reagent with tris（2,2-bipyridyl） ruthenium（Ⅱ） in the presence of acid.The existence of sodium sulfite made the reaction more sensitive and strong.The optimal conditions for the CL detection were studied and a new method for determining sodium sulfite was described by HPLC-CL.The linear range was 2.0×10-6~5.0×10-4g/mL with the detection limit of 6.0×10-7g/mL,linear regression equation：△/=3.578c＋19.721（c：g/mL;r2=0.9984）,and the relative standard detection was 3.7% for 5.0×10-5g/mL.This method has obtained satisfactory results in the analysis of sodium sulfite in beer samples.

关 键 词：高效液相色谱 化学发光 钌联吡啶 硫酸铈 亚硫酸钠 

分 类 号：TS207.3[轻工技术与工程—食品科学]