紫色马铃薯芽快繁技术研究  

Study on the Hormone of Micropropagation Technology of Purple Potato Bud

作　　者：汪云 罗富英[2] 李典[2] 李路金[2] 蓝素华[2] 

机构地区：[1]广东省湛江市农业科学研究所,广东湛江524048 [2]湛江师范学院

出　　处：《现代农业科技》2012年第4期151-152,共2页Modern Agricultural Science and Technology

基　　金：广东省科技攻关项目:粤科规划字[2009]196号(2009B020310017);粤科规划字[2009]188号(2009B0203100)

摘　　要：以幼嫩紫色马铃薯芽为试验材料,探讨不同激素组合对紫色马铃薯芽快速繁殖生长情况的影响,并成功获得一批无菌苗。结果表明:以75%酒精(CH3CH2OH)、0.1%升汞分别灭菌10 s、8 min,外植体诱导出芽数最高可达23个,长势良好。紫色马铃薯芽诱导培养基为MS+0.5 mg/L 6-BA+0.3 mg/L NAA+30 g/L蔗糖+7 g/L琼脂,芽分化成苗率为57.50%,茎粗壮,长势整齐,苗高5.12 cm,生根率达60.00%。In this experiment,purple potato（Solanum tuberosum L.）was used as the experimental material.Studing on the concept of micropropagation of purple potato bud expant,and some cultural plantlets were obtained successfully.The experimental results indicated that separately using 75% CH3CH2OH（8 min） and 0.1% HgCI2（10 s） to sterilize the loseeonds,the average number of buds up to 23,and the buds grow well.The buds induction medium of purple potato was MS＋0.5mg/L 6-BA ＋0.3 mg/L NAA ＋30 g/L sucros ＋7 g/L agar.The rate of buds differentiation was 57.50%,the plantlets growed thick and strong,and the sprout growed evenly.The height of plant was 5.12 cm,and the rate of root growing reached to 60.00%.

关 键 词：紫色马铃薯 幼芽 快速繁殖 外源激素 

分 类 号：S532[农业科学—作物学]