白细胞介素1β对失神经肌肉保护作用的实验研究  

Experiment study on protective effect of interleukin-1β on denervated muscle

作　　者：王全震[1] 万圣祥[1] 肖颖锋[1] 王拥军[1] 周喆刚[1] 王发斌[2] 

机构地区：[1]北京大学深圳医院手外科,广东深圳518036 [2]华中科技大学同济医学院附属协和医院手外科,武汉430022

出　　处：《重庆医学》2012年第6期532-534,F0002,共4页Chongqing medicine

摘　　要：目的研究白细胞介素1β(IL-1β)对失神经支配骨骼肌的作用。方法将36只健康SD大鼠制备失神经支配腓肠肌模型,随机分成实验组和对照组,每组18只。A组于腓肠肌不同的5个点注射IL-1β0.1mL(浓度1ng/mL),B组注射同等剂量的生理盐水,术后每天注射1次。于术后2、4、8周各取6只大鼠处死,取右侧腓肠肌观察,测定肌湿质量维持率、肌细胞横截面积、肌肉蛋白总含量,检测运动终板,细胞凋亡等指标。结果术后2、4、8周实验组的肌湿质量维持率、肌细胞截面积、肌肉蛋白含量均明显高于对照组(P<0.05)。术后8周两组的运动终板平均灰度值和平均光密度值差异有统计学意义(P<0.05)。实验组肌细胞凋亡率明显低于对照组,差异有统计学意义(P<0.05)。结论靶肌肉局部注射IL-1β能减轻失神经骨骼肌形态的改变,对失神经骨骼肌和运动终板具有保护作用,能在短时间内有效延缓失神经支配骨骼肌萎缩。Objective To study the protective effect of interleukin-1β（IL-1β） on denervated skeletal muscle.Methods A model of denervated gastrocnemius at right lower limb was established in 36 healthy adult SD rats.The rats were randomly divided into two groups： experiment group and control group.The IL-1β（1 ng/mL） with 0.1mL was directly injected into the denervated gastrocnemius of the experimental group at 5 different spots every day,and the isodose physiological saline was directly injected into the denervated gastrocnemius of the control group with the same method.On postoperative 2,4,8 weeks,6 rats were taken from the two groups,the rate of muscle wet weight preservation,the cross section area of myocyte,the protein amount and the motor end-plate were checked respectively for investigating the protective effect of interleukin-1β on denervated skeletal muscle.Results On postoperative 2,4,8 weeks,the rate of muscle wet weight preservation,the cross section area of myocyte and the protein amount were significantly greater in the experiment group than those in the control group（P0.05）.On postoperative 8 weeks,the edge of motor end-plate in the experimental group was more distinct than that in the control group,and the acetylcholine esterase dyeing of motor end-plate was deeper than the control group.The percentage of apoptotic muscle cells was significantly smaller in the experiment group than that in the control group.Conclusion IL-1β can retard denervated muscle atrophy in short period.

关 键 词：白细胞介素1Β 失神经支配 肌萎缩 

分 类 号：R746[医药卫生—神经病学与精神病学]