生物法提取杜仲胶菌株筛选及发酵条件优化  

Screening Strains for Extraction of Rubber from the Bark of Eucommia ulmoides and Optimization of Fermentation Conditions

作　　者：丁奋霞[1] 苏印泉[1] 杜双田[2] 张强[1] 中泽庆久[3] 堤雅史[3] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]西北农林科技大学生命科学学院,陕西杨陵712100 [3]大阪大学

出　　处：《西北林学院学报》2012年第2期149-154,共6页Journal of Northwest Forestry University

基　　金：中日(NEDO)合作项目(K332020908);陕西省日元贷款项目(K332020023)

摘　　要：采集杜仲老林区的土壤和腐殖质,以杜仲皮作为基质多次富集培养,分离其优势菌,通过测定其发酵性能指标并建立菌株综合选择指数,筛选生物法提取杜仲胶的优良菌株,并研究优良菌株的发酵适宜条件。筛选出了优良菌株P-24,该菌株具有降解能力强,发酵周期短,杜仲胶的发酵损失率小等优点,其适宜的发酵温度为30℃、基质含水率65%、初始pH7.0,在此条件下发酵12d,杜仲皮的发酵物去除率可达93.02%,发酵剩余物中杜仲胶的含量为74.71%,杜仲胶的得率为5.25%。筛选优良菌株是生物法提取杜仲胶的关键;菌株P-24在实际生产中具有一定的应用价值。Strains in the soil and humus samples from Eucommia ulmoides forest were screened for the formentation of E.ulmoides bark and for the extraction of Eucommia rubber.Then predominant strain was isolated.Fermentation conditions of predominant strain was optimized.The results showed that strain P-24 was efficient strain with stronger capacity to degrade Eucommia bark with short fermentation period,low loss rate of the rubber.The optimal conditions of strain P-24 for fermentation may get when the medium moisture content was 65%,initial pH was 7.0,fermentation temperature was 30 ℃,fermentation time was 12 d,and fermentation removing rate was 93.02%,rubber content was 74.71% after the bark was formented,and yield rate of rubber was 5.25%.

关 键 词：杜仲胶 菌株筛选 固体发酵 发酵降解率 

分 类 号：S789.2[农业科学—林学]