变形链球菌耐氟菌株的诱导及产酸性的实验研究  

Induction of fluoride resistant mutant of S mutans and the measurement of its acidogenesis in vitro

作　　者：盛江筠[1] 刘正[1] 

机构地区：[1]上海第二医科大学口腔医学院口腔内科,200011

出　　处：《中华口腔医学杂志》2000年第2期95-98,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目 !(39870780)

摘　　要：目的　探讨变形链球菌耐氟菌株致龋毒力的变化。方法　在含有不同氟浓度的TSA上逐步诱导变形链球菌 (S mutansIngbritt)产生耐氟菌株Ingbritt FR ,用气相色谱法检测耐氟菌株的产酸量及降低pH值的能力 ,并与亲代野生菌株进行比较。结果　Ingbritt FR在体外诱导成功并稳定传代培养 ;当氟化物存在时 ,耐氟菌株的产酸量大于亲代野生株 ;无氟化物存在时 ,初始pH≥ 5 5时其产酸量小于亲代株 ,pH <5 5时则大于亲代株 ,差异有显著性。结论　变形链球菌耐氟菌株具有致龋性 ;在菌斑正常氟浓度情况下 ,其致龋力可能大于亲代野生株。Objective To induce fluoride resistant mutant of S mutans in vitro and find out the differences of cariogenicity between them Methods Fluoride resistant strain Ingbritt FR was obtained by subculturing S mutans Ingbritt to TSA and TSB containing different concentrations of fluoride Lactic production and terminal pH of media were measured by gas chromatography method Results ①The stable fluoride resistant mutant of S mutans Ingbritt had been induced successfully in vitro; ② Lactic production of Ingbritt FR was significantly more than its parent strain at various initial pH values with 0 5 and 5 0 mmol/L fluoride whereas when no fluoride existed, there were two situations: the acid production was more than S mutans Ingbritt at initial pH above 5 0 while the result was opposite when pH was 5 0 and below Conclusion The fluoride resistant strain Ingbritt FR had a stronger cariogenic potential than its parent strain S mutans Ingbritt in physiological plaques

关 键 词：变形链球菌 龋齿 耐氟菌株 产酸量 

分 类 号：R780.2[医药卫生—口腔医学]