高糖高脂诱导胰岛素抵抗HepG2细胞模型的建立及活性成分的功能评价  

Establishment of the insulin resistance HepG2 cell model with elevated levels of glucose and free fatty acid and functional evaluation of active compounds

作　　者：柳嘉[1] 郭孝萱[2] 吴薇[3] 高丰衣[1] 王鸥[1] 贾晓楠[1] 周峰[1] 籍保平[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]中国农业大学生物学院,北京100193 [3]中国农业大学工学院,北京100083

出　　处：《食品科技》2012年第3期73-78,82,共7页Food Science and Technology

摘　　要：胰岛素抵抗是导致Ⅱ型糖尿病发生的主要机制之一,它会导致高血糖,最终引发糖尿病发病。目前胰岛素抵抗的细胞模型多样,但是以单一因素诱导造模为主。利用高糖联合高脂诱导HepG2细胞模型,更符合人体发病时高血糖高血脂的状态。实验采用25mmol/L葡萄糖联合不同浓度的油酸软脂酸(摩尔浓度比2:1),于36h测定细胞的糖吸收、糖原含量以及甘油三酯含量,以确定合理的造模浓度。最终用0.5mmol/L的游离脂肪酸联合诱导液建立了胰岛素抵抗HepG2细胞模型,并利用此模型对4种成分改善胰岛素抵抗进行了评价。结果发现,葛根素、甜菊苷、熊果酸和表儿茶素均能有效地改善HepG2细胞模型的胰岛素抵抗,相比较甜菊苷和表儿茶素的效果较好。Insulin resistance is one of the main mechanisms of type Ⅱdiabetes, it leads to hyperglycemia, and ultimately leads to diabetes. The current insulin-resistance cell models are mainly single factorinduced models. Induced HepG2 cell model using elevated glucose and free fatty acids seems more in accordance with the high cholesterol and hyperglycemia status of human body when the disease occurs. To determine the reasonable modeling condition, cells were induced by 25 mmol/L glucose combined with different concentrations of oleic acid and palmitic acid （molar ratio 2：1） for 36 h, later the glucoseabsorption, glycogen and triglyceride levels were tested. HepG2 cell model of insulin resistance was established with 0.5 mmol/L free fatty acids, and four components were evaluated. The results showed that puerarin, stevioside, ursolic acid and epicatechin could effectively alleviate the insulin resistance. Moreover, stevioside and epicatechin had better effects.

关 键 词：胰岛素抵抗 HEPG2细胞 葛根素 甜菊苷 熊果酸 表儿茶素 

分 类 号：R282.71[医药卫生—中药学]