金纳米粒子比色探针检测牛奶及鸡蛋中的三聚氰胺  

Gold Nanoparticles-based Colorimetric Sensing of Melamine in Milk and Eggs

作　　者：孙春燕[1] 张民伟[1] 李宏坤[1] 李岩松[1] 平红[1] 郭佳佳[1] 张铁华[1] 

机构地区：[1]吉林大学军需科技学院,长春130062

出　　处：《分析化学》2012年第3期386-390,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20905031);吉林大学引进优秀人才科研基金(No.430505010206H6)资助项目

摘　　要：三聚氰胺能诱导金纳米粒子(AuNPs)团聚,溶液颜色由酒红色变为紫色或蓝灰色。以AuNPs作为比色探针,建立了快速检测牛奶和鸡蛋中三聚氰胺的方法。实验优化得最佳反应条件为:AuNPs粒径13min、pH=7、反应时间10 min和温度为室温。对样品中常见物质进行了干扰实验。样品经10%三氯乙酸和氯仿提取、离心分离后可直接测定,本方法对牛奶和鸡蛋中三聚氰胺的检出限分别为0.1和2.5 mg/L(S/N=3),加标回收率分别为95.0%～105.0%和98.0%～104.0%。Melamine could rapidly induce the aggregation of gold nanoparticles（AuNPs）,thereby resulting in red-to-purple（even blue） color change.Thus,a rapid detection method for melamine in milk and eggs using AuNPs as colorimetric probe has been established.The experimental conditions was optimized in detail,ultimately,the analysis was carried out using 13 nm AuNPs in the media of pH 7,and the reaction was perfomed for 10 min at room temperature.Moreover,the effects of interfering substances in the samples were investigated.The melamine can be detected after extraction and centrifugation separation with 10% trichloroacetic acid and chloroform.The proposed method could be used to detect melamine in milk and eggs with a detection limit of 0.1 mg/L,2.5 mg/L（S/N=3）,and the recovery are 95.0%-105.0%,98.0%-104.0% respectively.

关 键 词：三聚氰胺 金纳米粒子 比色 牛奶 鸡蛋 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]