Fmr1基因敲除小鼠的ABR阈值观察  

Auditory brainstem response in FMR-1 knockout mice

作　　者：方敏华[1] 马钊恩[1] 孙卫文[1] 黄月玲[2] 沈岩松[1] 戴丽军[2] 陈盛强[1] 张建国[1] 李敏雄[1] 

机构地区：[1]广州医学院附属第二医院,广东广州510260 [2]广州医学院动物实验中心,广州510182

出　　处：《解剖学研究》2012年第1期30-34,共5页Anatomy Research

基　　金：广东省自然科学基金(815101700100005);广东省科技计划项目(2008B030301371;2009B030801368;2009B060300018);广东省中医药管理局建设中医药强省科研基金(2010206)

摘　　要：目的对不同周龄的KO与WT小鼠听阈进行检测并对比,了解KO小鼠的听阈变化。方法采用PCR法鉴定FMR1基因敲除型(KO)纯合子(-/-)及其野生型(WT)纯合子(+/+)FVB近交系小鼠,实验动物150只分两组:(1)KO组(3、4、6、8、10周龄,每周龄15只,共75只;②WT组(3、4、6、8、10周龄,每周龄15,共75只,用于听性脑干反应(ABR)测试。数据及图像的采集:以ABR图形中Ⅱ波的阈值为小鼠的ABR阈值。结果 ABR阈值:3周及4周年龄组小鼠各基因型间KO小鼠的ABR阈值显著高于WT小鼠,差异有统计学意义(P<0.01);6、8、10周各组中各基因型小鼠的KO小鼠和WT小鼠的ABR阈值的差异无统计学意义(P>0.05)。结论幼年期FMR-1 KO小鼠听阈提高,成熟期FMR-1 KO小鼠听阈无异常,KO小鼠ABR的结果与AGS发生的年龄依赖性相一致。Objective To identify Fmr1 knockout mice,the ABR threshold was examined and compared to wild type at different ages.Methods Experimented animals FVB strain mice,Fmr-1 knockout（FMR-/-） and their wild type（FMR＋/＋） counterparts were used.They were genotyped by PCR to confirm the lack of the FMR-1 gene.Experimented animals were divided into 5 groups according to their genotype and age： KO group（3weeks,4weeks,6weeks,8weeks,10weeks） and WT group（3weeks,4 weeks,6 weeks,8 weeks,10 weeks）,each group has 30 FVB mouse.ABR was detected after anesthesia.Results ABR threshold of KO group（3 weeks,4 weeks） was increased remarkably compared with the control group（P0.01）.There were no differernce between KO group and WT group in 6 weeks,8w and 10 weeks,respectively（P0.05）.Conclusion ABR threshold has significantly difference between immature KO mice and immature WT mice.ABR threshold has no significantly difference between mature KO mice and mature WT mice.

关 键 词：脆性X染色体综合征 家族性智力低下基因-1 FMR1基因敲除小鼠 听性脑干反应 

分 类 号：R749.94[医药卫生—神经病学与精神病学]