橘林油脂酵母发酵条件的初步研究  

Investigation of fermentation conditions for microbial oil production by Lipomyces kononenkoae

作　　者：张东辉[1] 梁焯[1,2] 万霞[1,2] 龚阳敏[1] 江木兰[1,2] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2012年第1期82-86,95,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家863计划(2011AA100904);国家自然科学基金(30970262)

摘　　要：以橘林油脂酵母(Lipomyces kononenkoae CICC1714)为实验材料,通过设计单因素实验,确定了摇瓶发酵培养的最佳产油条件为:氮源为硫酸铵,碳源为葡萄糖,培养温度28℃,接种量10%,初始pH值为7.0,最适碳氮比为99。优化后批次发酵的生物量为7.01g/L,油脂含量15.91%,油脂产量1.12g/L。经半连续发酵后最终的生物量为24.13g/L,油脂产量为3.06g/L。The oil-producing conditions of Lipomyces kononenkoae were optimized on flask fermentation level by the single-factor experiment.The highest lipid yield was obtained with ammonium sulfate and glucose as nitrogen source and carbon source,respectively.The optimum culture conditions for temperature,inoculation,initial pH value and C/N ratio were 28℃,10%,7.0 and 99,respectively.Batch culture result showed that the biomass,lipid content and lipid yield of Lipomyces kononenkoae could reach 7.01g/L,15.91%,1.12g/L respectively after optimization.The results of flask fed-batch cultures indicated the dry biomass and lipid yield of Lipomyces kononenkoae could further reach 24.13g/L and 3.06g/L respectively.

关 键 词：橘林油脂酵母 单因素实验 发酵条件 微生物油脂 

分 类 号：Q93[生物学—微生物学]