7种常见食用菌中多糖的提取和测定  

Extraction and Determination of Polysaccharides from Seven Kind of Common Edible Fungus

作　　者：李承范[1] 

机构地区：[1]延边大学分析测试中心,吉林省延吉市133002

出　　处：《光谱实验室》2012年第2期996-999,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：吉林省教育厅科技计划项目:吉教科合字[2009]14号

摘　　要：采用热水提取、乙醇沉淀的方法从7种食用菌中提取出了水溶性多糖,采用蒽酮-硫酸法在波长620nm处进行了糖含量的测定,求得其校准曲线的回归方程为y=0.0094x+0.0057,相关系数r为0.9998。平均回收率为98.3%—102.7%,RSD为2.1%—3.2%。结果显示粗多糖的提取率为4.44%—16.75%,多糖含量为3.48%—6.91%。Hydrosoluble polysaccharides were extracted from seven kinds of common edible fungus by hot water-ethanol precipitation method.The contents of polysaccharides were determined by anthrone-sulfuric acid method at wavelength of 620nm,thus the regression equation of calibration curve was y=0.0094x＋0.0057 with correlation coefficient of 0.9998.The average recoveries were 98.3%—102.7% with relative standard deviations（RSDs） of 2.1%—3.2%.The extraction efficiencies of polysaccharides were 4.44%—16.75%,and the contents of polysaccharides were 3.48%—6.91%.

关 键 词：食用菌 水溶性多糖 提取 蒽酮-硫酸法 

分 类 号：O657.32[理学—分析化学]