顶空固相微萃取-气相色谱-质谱联用分析马蹄香中的挥发油成分  

Analysis of the Essential Oil from Saruma henryi Oliv by HS-SPME-GC-MS

作　　者：王艳[1] 娄方明[2] 杨建文[2] 蔡义云[2] 

机构地区：[1]湖北民族学院化学与环境工程学院,湖北省恩施市学院路39号445000 [2]遵义医学院药学院,贵州省遵义市大连路201号563000

出　　处：《光谱实验室》2012年第2期1076-1080,共5页Chinese Journal of Spectroscopy Laboratory

摘　　要：利用顶空固相微萃取-气相色谱-质谱联用技术分析马蹄香挥发油的化学成分,为马蹄香的挥发油成分分析提供新方法。以顶空固相微萃取法提取挥发油,气相色谱-质谱联用技术对挥发油成分进行分离鉴定,并采用面积归一化法确定各成分的相对质量分数。结果样品在90℃下平衡30min,顶空吸附15min的条件下,采用100μm PDMS纤维头能有效地吸附马蹄香挥发油成分。气相色谱-质谱联用技术共鉴定出57个成分,其中相对质量分数较高的有西车烯(15.93%)、1,2,3,5,6,7,8,8a-八氢-1,4-二甲基-7-(1-甲基乙烯基)甘菊蓝(13.55%)、α-广藿香烯(9.50%)等。马蹄香挥发油具有丰富的化学成分,顶空固相微萃取-气相色谱-质谱联用能全面快速地获得其组成信息,可应用于马蹄香挥发油成分的研究。A new method was established to analyse the compounds of essential oil from Saruma henryi Oliv by headspace-solid phase microextraction（HS-SPME） and identify them by gas chromatography-mass spectrometry（GC-MS） technology.The relative mass fraction of essential oil compounds were got by area normalization method.The essential oil compounds from Saruma henryi Oliv can be effectively extracted by the fiber of 100μm PDMS at 90℃ under the conditions of equilibrium for 30min and headspace adsorption of 15min.57 compounds were identified.The main constituents of the oil were seychellene（15.93%）,azulene,1,2,3,5,6,7,8,8a-octah-ydro-1,4-dimethyl-7-（1-methylethenyl）-（13.55%）,α-patchoulene（9.50%） et al.There were abundent chemical constituents in the essential oil of Saruma henryi Oliv.HS-SPME-GC-MS can entirely and quickly obtain the information about the constituents of essential oil from Saruma henryi Oliv,and can be applied for analysis of essential oil from Saruma henryi Oliv.

关 键 词：马蹄香 挥发油 顶空固相微萃取 气相色谱-质谱联用 

分 类 号：O657.63[理学—分析化学]