IL-2Rα结合位点修饰型OviIL-2在毕赤酵母中的表达与鉴定  

Expression and Identification of a IL-2Rα Binding Sites Modified roviIL-2 in Pichia Pastoris

作　　者：王文昊[1,2] 苗利光[2] 李海涛[2] 刘艳环[2] 王松[2] 安亚雄[1,2] 冯卓[2] 

机构地区：[1]江苏科技大学,江苏镇江212018 [2]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2012年第1期12-13,51,共3页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20100219)

摘　　要：IL-2主要由激活后的T细胞产生,是一种天然的免疫增强剂,临床应用广泛。之前构建了适用于毕赤酵母表达系统的重组绵羊IL-2的真核表达载体pPIC9K-roIL2。本研究在此基础上使用pPIC9K-roIL2转化毕赤酵母GS115,筛选得到阳性克隆,命名为GS115-roIL2并经过摇瓶培养和诱导表达得到表达产物。通过SDS-PAGE进行初步鉴定,证明成功表达出了roIL-2。本研究为下一步roviIL-2免疫机理研究奠定了基础。IL2 is mainly produced by activated T lymphocyte, act as a natural immune enhancer, and have been widely us- ing in the clinical setting. In previous study we constructed a recombinant ovine IL2 expression vector pPIC9KroIL2 apply to Pichia pastoris eukaryotic expression system. PPIC9KroIL2 was transformed into Pichia pastoris GS115, and transformation clones were selected and named as GS115rolL2. Products were harvested after incubation and induction in flask. Latterly the products were identified by SDSPAGE. It showed that roIL2 was successfully expressed, and this would provide basis for the further study of immune mechanism of roviIL2.

关 键 词：绵羊白细胞介素2 毕赤酵母 诱导表达 

分 类 号：Q786[生物学—分子生物学]