菜籽降压肽双酶水解液脱色工艺的研究  

Discoloring Technique of Antihypertensive Peptides from Rapeseed Hydrolyzed by Double Enzymes

作　　者：张艳[1] 刘志伟[1] 

机构地区：[1]武汉工业学院食品科学与工程学院,武汉430023

出　　处：《生物技术通报》2012年第3期186-190,共5页Biotechnology Bulletin

基　　金：国家"863"计划项目(2010AA023004);武汉市科技创新基金项目(2009028)

摘　　要：用活性炭对菜籽降压肽双酶水解液进行脱色处理,采用单因素试验以及单因素试验基础上的正交试验法。考察活性炭用量、pH、温度、时间对水解液脱色率、肽损失率、活性及ACE抑制率的影响。正交试验优化结果表明,菜籽降压肽双酶水解液脱色最佳工艺为活性炭的用量为1%、脱色pH值为3.0、脱色温度为80℃、脱色时间为50 min,在此条件下脱色率为85.52%、ACE抑制率为57.83%、活性损失率为5.69%。Antihypertensive peptides from rapeseed,which hydrolyzed by double enzymes,was discolored by activated carbon,and by using the single factor and orthogonal design method.The factors including the quantity of activated carbon,pH,temperature and time were investigated in terms of discoloring efficiency,peptide loss,angiotensin-converting enzyme inhibitory activity（ACEI）.The result shows that the optimum discoloration conditions are as follows： quantity of activated carbon 1%,pH3.0,temperature 80℃,time 50 minutes.Under these conditions,the discoloring efficiency of hydrolyzate,ACEI,peptide loss,were 85.52%,57.83% and 5.69%.

关 键 词：菜籽降压肽 酶解液 脱色 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]