树脂柱色谱纯化甘油磷脂酰胆碱  

Purification of L-α-glycerylphosphorylcholine using resin column chromatography

作　　者：韩山山[1] 张康逸[1] 黄健花[1] 刘元法[1] 王兴国[1] 

机构地区：[1]江南大学食品学院,食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《中国油脂》2012年第3期47-51,共5页China Oils and Fats

基　　金：国家高技术研究发展计划(863计划)(2010AA101506)

摘　　要：研究了树脂柱色谱纯化甘油磷脂酰胆碱(L-α-GPC)的可行性,通过树脂筛选,确定D001大孔阳离子交换树脂为最佳纯化树脂,并对纯化工艺条件进行了优化。通过静态实验得到最优条件:上样质量浓度1.52 mg/mL,样品溶液pH为7,吸附时间300 min,解吸液为去离子水。在静态实验的基础上,进行了树脂柱色谱纯化L-α-GPC的动态实验,对吸附流速、解吸液和解吸流速进行优化。动态实验最优条件:吸附流速2 mL/min,解吸液为去离子水,解吸流速2 mL/min。依据动态实验最优条件,最终L-α-GPC的纯度为96%,回收率为54.1%,这表明树脂柱色谱纯化L-α-GPC的方法是经济、方便、可行的。The feasibility of using resin column chromatography to purify the L-α-glycerylphosphorylcholine（L-α-GPC） was studied.Through the screening of resins,the D001 macroporous cation resin was the best one,and it was used to optimize the purification conditions.Through the static test,the optimal conditions were as follows：the loading concentration 1.52 mg/mL,eluent deionized water,pH of sample solution 7,adsorption time 300 min.On the base of the static test,the dynamic test of the resin column chromatography purifying L-α-GPC was studied,and the conditions of the adsorption flow rate,desorption solution and desorption flow rate were optimized.The dynamic optimal conditions were as follows：adsorption flow rate 2 mL/min,eluent deionized water,elution flow rate 2 mL/min.According to the optimal dynamic test conditions,the purity and recovery of L-α-GPC was 96% and 54.1%,respectively,which indicated that using resin column chromatography to purify L-α-GPC was economic,convenient and practical.

关 键 词：树脂柱色谱 甘油磷脂酰胆碱 纯化 最优条件 

分 类 号：TQ464[化学工程—制药化工]