坐骨神经夹伤与心脏毒素注射导致腓肠肌功能损伤中Calpains和FoxOs的差异表达及相关性分析  

The mRNA alteration and correlation of Calpains and FoxOs during gastrocnemius muscle atrophy induced by sciatic nerve injury and cardiotoxin injection

作　　者：巫荣华[1] 徐曼[1] 赵攀峰[1] 刘梅[1] 

机构地区：[1]南通大学神经再生重点实验室,江苏省南通市226001

出　　处：《中国组织工程研究》2012年第7期1173-1179,共7页Chinese Journal of Tissue Engineering Research

基　　金：教育部科学技术研究重点项目(208044);南通大学创新课题项目(YKC09050);江苏高校优势学科建设工程资助项目(PAPD)~~

摘　　要：背景:钙蛋白酶家族和FoxO转录因子在肌萎缩过程中发挥着重要的作用。目的:观察钙蛋白酶家族成员及FoxO转录因子(FoxO1和FoxO3a)在心脏毒素注射与坐骨神经夹伤致腓肠肌失功能损伤中表达的变化。方法:分别采用坐骨神经夹伤和心脏毒素注射建立大鼠腓肠肌功能损伤模型,于造模后的第3,7,14,21天取大鼠腓肠肌进行实时荧光定量PCR检测,并采用线性回归法进行相关性分析。结果与结论:结果显示,腓肠肌组织中的钙蛋白酶Ⅰ、钙蛋白酶Ⅱ和FoxO3amRNA在坐骨神经夹伤7d达到高峰,随后下降;钙蛋白酶Ⅲ和FoxO1mRNA在坐骨神经夹伤后14d达到高峰,随后下降。钙蛋白酶Ⅰ、钙蛋白酶Ⅱ、FoxO3amRNA在心脏毒素注射后第3天达高峰,随后逐渐下降;FoxO1mRNA在心脏毒素注射后7d时达最高;而钙蛋白酶ⅢmRNA在心脏毒素注射后3d时表达显著下降,随后逐渐恢复至正常水平。线性回归分析结果显示,在失神经肌损伤过程中钙蛋白酶Ⅲ的表达与FoxO1呈正相关(r=0.9015);在心脏毒素注射肌损伤过程中钙蛋白酶Ⅰ(r=0.8987)和钙蛋白酶Ⅱ(r=0.9374)表达趋势与FoxO3a呈正相关。可见,在不同的肌损伤模型中,钙蛋白酶Ⅰ和钙蛋白酶Ⅱ的表达趋势相似,而钙蛋白酶Ⅲ的表达不同。BACKGROUND：The calpain family members（Calpains） and FoxO transcription factors（FoxOs） play important roles during muscle atrophy.OBJECTIVE：To investigate the different expression patterns and correlations of Calpains（Calpain 1,Calpain 2,Calpain 3） and FoxO transcription factors（FoxO1 and FoxO3a） during gastrocnemius muscle atrophy induced by sciatic nerve injury and cardiotoxin injection.METHODS：The gastrocnemius muscle atrophic models were established by sciatic nerve injury and cardiotoxin injection.The mRNA level of Calpains and FoxOs were detected by real time quantitative PCR at 3,7,14 and 21 days after injury.The linear regression analysis was performed to conduct the correlations between Calpains and FoxOs.RESULTS AND CONCLUSION：The expression level of Calpain 1（CAPN1）,Calpain 2（CAPN2） and FoxO3a mRNA reached to peak at 7 days after sciatic nerve injury,and then decreased gradually;the mRNA level of Calpain 3（CAPN3） and FoxO1 reached to peak at 14 days after sciatic nerve injury,then declined.After cardiotoxin injection,the expression level of CAPN1,CAPN2 and FoxO3a mRNA rapidly reached to peak at 3 days after cardiotoxin injection and then slowly went back down;the mRNA level of FoxO1 found its climax at 7 days after cardiotoxin injection,while that of CAPN3 instantly got to its lowest point at 3 days after cardiotoxin injection.The result of linear regression analysis showed that there was a significant positive correlation between the expression of CAPN3 and FoxO1 mRNA（r=0.901 5） during gastrocnemius muscle atrophy induced by sciatic nerve injury;And after cardiotoxin injection,the strong positive correlation existed between the mRNA level of CAPN1,CAPN2 and that of FoxO3a（r=0.898 7,r=0.937 4） respectively.The present study indicated that the mRNAs alteration of CAPN1 and CAPN2 was similar in the two different muscle injury models,while CANP3 displayed a different expression pattern.

关 键 词：坐骨神经夹伤 心脏毒素 肌萎缩 钙蛋白酶家族 FOXO1 FOXO3A 

分 类 号：R318[医药卫生—生物医学工程]