原发性NSCLC中3p杂合性丢失的研究  

Allele specific chromosome 3p deletion in primary non small cell lung cancer

作　　者：关赛芳[1] 许凯黎[1] 赵明[1] 陈复华[1] 周瑾[1] 

机构地区：[1]上海市肿瘤研究所,200032

出　　处：《中国肺癌杂志》2000年第1期20-22,共3页Chinese Journal of Lung Cancer

基　　金：上海市科技发展基金项目资助! (96451 2 0 0 3)

摘　　要：目的　探讨非小细胞肺癌 (NSCLC)中第 3号染色体短臂 ( 3p)上两个独特区域——— 3p14和3p2 5等位基因的杂合性丢失 (LOH)与NSCLC发生、发展之间的关系。方法　采用聚合酶链反应 (PCR)结合二核苷酸 (CA)n重复序列多态性方法 ,分析 162例新鲜NSCLC手术标本、85例癌旁肺组织及 5例肺部良性疾病组织中 3p14和 3p2 5位点的LOH。结果　 162例NSCLC组织中共有 10 8例 ( 66.67% )出现LOH ,84例 ( 5 1.85 % )为 3p2 5LOH ,65例 ( 4 0 .12 % )为 3p14LOH ,其中 41例 ( 2 5 .3 1% )同时出现 3p14和 3p2 5两个位点的丢失。 3pLOH与肺癌组织学分类及TNM分期均无明显关系。但腺癌中 3 8% ( 19/5 0 )同时出现3p2 5和 3p14LOH ,明显高于鳞癌 ( 19.5 1% ,16/82 ) (P <0 .0 5 )。而且发生 3p14LOH的Ⅱ期NSCLC患者( 5 1.2 8% ,2 0 /3 9)和Ⅲ期患者 ( 4 4.93 % ,3 1/69)均明显高于Ⅰ期 ( 2 5 .93 % ,14 /5 4) (P均 <0 .0 5 )。 85例癌旁肺组织及 5例肺部良性疾病组织均未发现 3pLOH。结论　 3p杂合性丢失普遍存在于NSCLC中。在这些丢失区域内可能存在一个或多个抑癌基因 。Objective To investigate the relationship between loss of heterozygosity (LOH) of the allele genes on 3p and the pathogenesis of NSCLC. Methods Using polymerase chain reaction based assays for dinucleotide repeat polymorphisms to analyze allele loss of 3p in 162 NSCLC samples. Results Of the 162 cases with NSCLC, 108 (66.67%) had LOH on 3p. Among them, 65 cases (40.12%) had LOH at 3p14, 84 cases(51.85%) had LOH at 3p25, and 41 cases (25.31%) occured co deletion of 3p14 and 3p25. No 3p LOH were observed in 85 paracancerous lung tissues and 5 tissues of the pulmonary benign diseases. The total frequency of LOH at 3p did not relate to TNM staging and pathological classification in NSCLC (P> 0.05 ). However, the co deletion rate of 3p14 and 3p25 was significantly higher in adenocarcinoma (38%, 19/50) than that in squamous cell carcinoma (19.51%,16/82) (P<0.05). And 3p LOH rate in stage Ⅱ(51.28%,20/39) and stage Ⅲ(44.93%,31/69) were both remarkably higher than that of stage Ⅰ(25.93%,14/54)(P< 0.05 ).Conclusion 3p LOH occur commonly in human NSCLC. It suggests that each region harbor one or more tumor suppressor genes, the inactivation of which might be critical for the oncogenesis and development of lung cancer.

关 键 词：非小细胞肺癌 杂合性丢失 染色体3P PCR-LOH 

分 类 号：R734.2[医药卫生—肿瘤]