酿酒酵母SNF4基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of SNF4 gene in saccharomycescerevisiae

作　　者：吴毕莎[1] 柯崇榕[1] 林晓华[1] 林清强[1] 陈由强[2] 黄建忠[1] 

机构地区：[1]福建师范大学工业微生物教育部工程研究中心生命科学学院福建省现代发酵技术工程研究中心,福州350108 [2]福建师范大学生命科学学院农业部甘蔗遗传改良重点开放实验室,福州350108

出　　处：《生物信息学》2012年第1期8-11,共4页Chinese Journal of Bioinformatics

基　　金：现代农业产业技术体系建设专项资金资助(No.nycytx-024-01-20);公益性行业(农业)科研专项项目(No.nyhyzx07-019);农业部948项目(No.2006-G37);福建省教育厅项目(No.JB10015)

摘　　要：SNF4基因编码的Snf4p具有调节Snf1复合体的蛋白激酶活性功能,根据已知的SNF4基因序列设计引物扩增获得S.cerevisiae YS2的SNF4基因完整序列。序列分析表明,SNF4基因的开放阅读框为969bp,编码322个氨基酸残基。应用生物信息方法预测其理化性质、疏水性、信号肽、亚细胞定位、活性位点及其高级结构。结果表明:Snf4p为具有一定亲水性的非跨膜胞内稳定酸性蛋白,功能结构域为CBS_pair superfamily结构域,二级结构主要由a-螺旋组成,空间结构是由4个CBS结构域构成两个CBS对围绕形成的二聚体。Snf4p的第一个CBS对区域的β片层结构是Snf1p、Sip2p的β发夹结构结合作用区。Snf4p is encoded by SNF4 gene which the function is regulating the protein kinase activity of Snf1 complex.Clone SNF4 gene from S.cerevisiae YS2 with primer which is designed by the known SNF4 sequence.Sequence analysis showed that SNF4 gene contains 969bp nucleotide acids,encoding 322 amino acids.Predict the physico-chemical property,hydrophobicity,signal peptide,subcellular localization,active site and spatial structure of SNF4 gene.The results show that Snf4p is the intracellular stability of acidic protein but does not exist transmembrane.The functional domain of Snf4p is CBS pair superfamily and the main secondary structure isα-helix.The spatial structure of Snf4p is dimer which is made by four CBS structural domains.Theβhairpin structure reaction region between Snf1p and Sip2p isβlamellar structure which is in the first CBS pair of Snf4p.

关 键 词：SNF4 酿酒酵母 ADH2 生物信息学 

分 类 号：TQ926.1[轻工技术与工程—发酵工程]