L-阿拉伯糖对2型糖尿病大鼠糖脂代谢的影响  

Effect of L-arabinose on Glucose and lipid metabolism in type 2 diabetic rats

作　　者：王鑫[1] 姚晓芬[2] 应茵[2] 刘静[3] 王竹[2] 向雪松[2] 易有金[1] 杨月欣[2] 

机构地区：[1]湖南农业大学食品科技学院,长沙410128 [2]中国疾控中心营养与食品安全所 [3]北京营养源研究所

出　　处：《卫生研究》2012年第2期260-263,267,共5页Journal of Hygiene Research

基　　金：国家"十二五"科技攻关项目(No.2012BAD33B01)

摘　　要：目的探讨L-阿拉伯糖对2型糖尿病模型大鼠血糖、血脂、脏器及胰岛的影响。方法采用雄性Wistar大鼠高糖高脂饲料喂养8周后,按25mg/kgbw的剂量一次性腹腔注射链脲佐菌素建立2型糖尿病动物模型,按L-阿拉伯糖低剂量(50mg/kgbw)、中剂量(150mg/kgbw)及高剂量(500mg/kgbw)进行灌胃,以阿卡波糖(20mg/kgbw)作为阳性对照,所用L-阿拉伯糖及阿卡波糖均配制于糊精(0.36g/ml)与蔗糖(0.04g/ml)的悬浮液中,自由进食进水。给药4周后进行口服葡萄糖耐量试验,处死后对大鼠肝脏、附睾脂肪及盲肠进行称重,腹主动脉取血测定TC、TG、血糖及胰岛素水平,通过免疫组织化学切片对胰岛β细胞损伤程度进行评价。结果与模型组对比,低、中、高剂量的L-阿拉伯糖干预对糖尿病大鼠的血糖应答产生了显著影响,30、60和120min血糖值及AUC均显著低于模型对照组(P<0.05),其中以中剂量效果最为显著(P<0.01)。L-阿拉伯糖的干预对糖尿病大鼠胰岛素敏感性的改善未见显著,但增加了盲肠重量,对胰岛β细胞也呈现出保护作用。本研究中L-阿拉伯糖的干预对于血脂和血胆固醇的影响较小。结论 L-阿拉伯糖的中长期干预能够改善2型糖尿病大鼠的糖耐量,这种作用可能与L-阿拉伯糖对食物消化酶的抑制及对胰岛β细胞的保护作用相关。Objective To investigate the effect of L-arabinose on glucose and Organ and islet in type 2 diabetic rats.Methods Male Wistar rats were fed high-sugar and high-fat diet after 8 weeks,then inject streptozotocin intraperitoneally according to 25mg/kgbw dose to establish animal models of type 2 diabetes,and fed L-arabinose according to a low dose（50mg/kgbw）,medium dose（150mg/kgbw） and high dose（500mg/kgbw）,the animal be fed acarbose（20mg/kgbw） as a positive control,the use of L-arabinose and acarbose were prepared in the dextrin（0.36g/ml） and sucrose（0.04g/ml） of the suspension,free to eat water.OGTT after 4 weeks,After animal death,rat liver,epididymal fat and cecum were weighed and calculate the ratio of organ weight.Results compared with model group,low,animals with L-arabinose-intervention those blood glucose response had a significant impact,30min,60min,120min blood glucose values and AUC were significantly lower than the model group（P0.05）,of which medium dose is most significant（P0.01）.L-arabinose intervention increased cecal weight and showed protective effect on β-cell.Conclusion L-arabinose long-term intervention can improve glucose tolerance in type 2 diabetic rats,this effect may be related to L-arabinose inhibition of digestive enzymes and the protective effect onβ-cell.

关 键 词：L-阿拉伯糖 2型糖尿病模型 改善糖耐量 保护胰岛β细胞 

分 类 号：R587.1[医药卫生—内分泌] Q591[医药卫生—内科学]