β-酪啡肽-7对大鼠血糖及脂质过氧化的影响  

Effect of β-Casomorphin-7 on Blood Glucose Level and Its Anti-lipid Peroxidation Effect in Diabetic Rats

作　　者：韩东宁[1] 王艳霞[1] 徐晨阳[1] 张源淑[1] 薛红霞[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,江苏南京210095

出　　处：《食品科学》2012年第5期243-247,共5页Food Science

基　　金：国家自然科学基金项目(30871838)

摘　　要：目的:观察β-酪啡肽-7(β-casomorphin-7,β-CM-7)对糖尿病大鼠的降血糖作用并初步阐明其作用机制。方法:2月龄SD雄性大鼠40只,用链脲佐菌素(STZ)致大鼠糖尿病,造模成功后将大鼠分为4个组:空白对照组、糖尿病对照组、胰岛素治疗组(优泌林3.7×10-8mol/(只.d))、实验组(β-CM-7 7.5×10-8mol/(只.d))。实验周期30d。测定血清中血糖含量、脂质过氧化物丙二醛(MDA)水平、超氧化物歧化酶(SOD)的活性、胰岛素和胰高血糖素的含量以及腿肌糖原、肝脏糖原的含量。结果:β-CM-7组与胰岛素治疗组大鼠血糖明显下降;同时血清中MDA水平降低,SOD活性升高,与糖尿病对照组相比,具有显著性差异(P<0.05);β-CM-7组胰岛素含量较糖尿病组有所增加,肌糖原含量较空白组显著升高(P<0.05)。结论:β-CM-7具有减轻链脲佐菌素性糖尿病大鼠氧自由基损伤、促进胰岛素分泌、加速肌糖原的合成、降低血糖的作用。Objective： To explore the effect of β-casomorphin-7 （β-CM-7） on blood glucose and oxidative stress in diabetic rats induced by streptozotocin （STZ）. Methods： Forty male SD rats were divided into 4 groups： normal group, diabetic control group, insulin treatment group （administered with humulin at 3.7 × 10^-8 mol/（day animal）） and β-CM-7 （7.5 × 10^-8 mol/（day animal）） treatment group. After administration for 30 consecutive days, all rats were sacrificed and measured for serum glucose level, MDA level, SOD activity and the contents of insulin and glucagons as well as leg muscle glycogen and liver glycogen contents. Results： The β-CM-7 treatment and insulin treatment groups revealed a significant decrease in blood glucose level, a reduction in MAD level, and an increase in SOD activity when compared with the diabetic control group （P 〈 0.05）. The β-CM-7 treatment group displayed an increase in insulin content when compared with the diabetic control group and a significant increase （P〈0.05） in liver glycogen content when compared with the blank control group. Conclusion： β-CM-7 can reduce free radical- mediated oxidative stress and blood glucose level, promote the secretion of insulin and increase the content of muscular glycogen in diabetic rats thus having hypoglycemic effect.

关 键 词：β-酪啡肽-7 大鼠 血糖 脂质过氧化 

分 类 号：R151.3[医药卫生—营养与食品卫生学]