多脂鳞伞紫外线诱变育种  

Ultraviolet mutation screening of Pholiota adipose

作　　者：解生权[1] 苏利[1] 陈伟[1] 朱琳[1] 马天卉[1] 全艳玲[2] 

机构地区：[1]辽宁经济职业技术学院生物工程系,辽宁沈阳110122 [2]辽宁科技大学化学工程学院,辽宁鞍山114055

出　　处：《中国酿造》2012年第3期66-68,共3页China Brewing

基　　金：辽宁省教育厅基金项目(L2010191)

摘　　要：该研究用已经选育出来的多脂鳞伞菌株作为出发菌株,利用紫外线对其孢子悬液进行诱变处理。结果表明,在紫外线照射65秒的情况下,得到一株正突变菌株(UV-65),经过斜面培养和发酵实验证实,其菌丝生长比出发菌株旺盛、菌丝产量提高20%;经过6代的连续传代培养,其遗传性状较为稳定。Using an obtained Pholiota adipose strain as starting strain,spore suspension was induced by ultraviolet（UV）.The results showed that a mutant strain UV-65 was obtained after 65s treatment under UV.It was demonstrated that UV-65 grew stronger than the starting strain and the production of mycelia increased by 20%.It had stable inheritance after six continuously passages.

关 键 词：多脂鳞伞 紫外诱变 菌种筛选 

分 类 号：Q93-331[生物学—微生物学]