九州虫草液体培养条件优化  

Optimum conditions of liquid culture of Cordyceps kyushuensis

作　　者：杜华东[1] 邵敬伟[1] 程显好[2] 林凤屏[1] 代永超[1] 

机构地区：[1]福州大学化学化工学院,福建福州350108 [2]鲁东大学生命科学学院,山东烟台264025

出　　处：《中国酿造》2012年第3期145-149,共5页China Brewing

基　　金：福建省科技厅青年人才创新项目(2007F0347);福建省教育厅项目(JA08004);福州大学科技发展基金(2009-XQ-12)

摘　　要：研究九州虫草富锌条件下菌丝体、胞内多糖和胞外多糖含量差异。通过单因子试验、正交试验测定不同因素的作用,菌丝体干重测定采用烘干称重法;胞内和胞外多糖测定应用苯酚-硫酸法和3,5-二硝基水杨酸法。结果表明,九州虫草在加锌条件下菌丝干重最优培养条件是葡萄糖2.0%,蛋白胨1.0%,磷酸二氢钾0.5%,硫酸镁0.5%,硫酸锌200mg/L,装液量80mL/250mL,pH值6.0。最终结果菌丝干重为1.53g/100mL,是原来未加锌基本培养基菌丝干重的2.58倍。The differences of mycelia biomass,intracellular polysaccharide and extracellular polysaccharide of Cordyceps kyushuensis were investigated under Zinc-enriched conditions in this paper.The optimal conditions were obtained by single factor and orthogonal tests as followed： sucrose 2.0%,peptone 1.0%,KH2PO4 0.5%,MgSO4 0.5%,ZnSO4 200mg/L,liquid medium volume 80ml/250ml and pH 6.0.Under these conditions,the biomass of mycelia was 1.53g/100ml,which was 2.58 fold higher than that without Zn conditions.

关 键 词：九州虫草 多糖 正交试验 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]