山茱萸籽总黄酮的响应曲面法优化微波辅助提取工艺的研究  

Optimization of microwave-assisted extraction of total flavonoids from Cornus officinalis seed by response surface methodology

作　　者：刘瑞林[1] 詹汉英[1] 张志琪[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室,陕西师范大学化学化工学院,陕西西安710062

出　　处：《食品工业科技》2012年第7期228-231,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(20875060)

摘　　要：目的:利用微波辅助提取技术对山茱萸籽总黄酮的提取工艺进行研究。方法:以芦丁为对照品,采用紫外-分光光度法测定山茱萸籽中总黄酮的含量,以总黄酮含量为考察指标,采用响应曲面优化法(RSM)优化山茱萸籽总黄酮的提取工艺。结果:优化的山茱萸籽总黄酮微波辅助提取工艺参数为:微波功率350W,乙醇体积分数50%,液料比20mL/g,提取时间13min。在此最佳条件下,山茱萸籽总黄酮的一次提取率为6.68%。结论:山茱萸籽含有较高的总黄酮含量,所确定的提取工艺提取率高、速度快、操作简便。Objective：A microwave-assisted extraction（MAE）method was studied for the extraction technology of total flavonoids from Cornus officinalis seed.Method：The total flavanoids contents of Cornus officinalis seed was determined by using ultraviolet-spectrophotometry method with rutin as reference substance.Using the content of total flavonoids in dry extract as a major evaluating factor,the best combination of extraction parameters was obtained with response surface methodology（RSM）.Results：The best extraction parameters were as follows：microwave power 350W;alcohol concentration 50%;solvent to sample ratio 20mL/g;extraction time 13min.Under these best conditions,the extraction yield of Cornus officinalis seed total flavonoids was 6.68%.Conclusion：The Cornus officinalis seed contain much higher total flavonoids content,and the technology had many advantages such as high yield,high speed,simple operation and so on.

关 键 词：微波辅助提取 山茱萸籽 总黄酮 响应曲面法(RSM) 

分 类 号：TS201.1[轻工技术与工程—食品科学]