离子色谱法测定玄参中的单糖和低聚寡糖  

Determination of monosaccharides and oligosaccharides in figwort root by ion chromatography

作　　者：熊建飞[1] 周光明[1] 何强[2] 许丽[1] 张磊 吴利敏[1] 

机构地区：[1]西南大学化学化工学院,发光与实时分析教育部重点实验室,重庆400715 [2]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《食品工业科技》2012年第7期340-343,共4页Science and Technology of Food Industry

基　　金：国家科技部重大专项项目(2008ZX07315)

摘　　要：建立阴离子交换色谱-脉冲安培检测法测定玄参中的葡萄糖、蔗糖、果糖和水苏糖的方法。以METROSEPCARB1(150mm×4.0mm)阴离子交换柱为分离柱,脉冲安培检测,30mmol/LNaOH溶液为淋洗液等度洗脱,超声水提法提取玄参中的糖。峰面积的相对标准偏差为0.56%～9.01%,线性范围1～50mg/L,检出限为0.0452～0.0921mg/L,相关系数R为0.9931～0.9998,加标回收率在96.52%～109.56%之间。该方法预处理简单,准确度高,适用于快速测定玄参中的单糖和低聚寡糖。An anion exchange chromatography-pulsed amperometric detection method has been proposed for determination of glucose,sucrose,fructose,stachyose in figwort root.The analytical column was METROSEP CARB 1（150mm×4.0mm）.The elution was performed using 30mmol/L sodium hydroxide isocratic elution and the column temperature was 32℃.A ultrasonoscope was used for extracting sugars.The RSD of area ranged from 0.56% to 9.01%,the linear range was 1~50mg/L,the detection limits was 0.0452~0.0921mg/L,the related coefficient（R）was 0.9931~0.9998,and the spike recoveries ranged from 96.52% to 109.56%.The method was simple and had a high sensitivity.So this method can be applied for the determination of monosaccharides and oligosaccharides in figwort root.

关 键 词：阴离子交换 脉冲安培 等度洗脱 超声水提 玄参 单糖 低聚寡糖 

分 类 号：TS207.3[轻工技术与工程—食品科学]