姜辣素对顺铂致大鼠异嗜模型行为的作用  

The effect of gingerol on cisplatin-induced pica in rats

作　　者：王茂龙[1] 杨玉玲[2] 魏朝霞[1] 岳旺[3] 徐万群[4] 

机构地区：[1]青岛大学医学院附属医院胸外科,山东青岛266003 [2]青岛大学医学院附属医院感染科,山东青岛266003 [3]青岛大学医学院药理学教研室,山东青岛266021 [4]青岛大学医学院附属医院急诊内科,山东青岛266003

出　　处：《中国药理学通报》2012年第4期558-562,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30873108/H3108);山东省自然科学基金资助项目(No Y2007C097)

摘　　要：目的研究姜辣素对顺铂所致大鼠异嗜高岭土模型的影响并观察外周和中枢c-fos蛋白的表达。方法大鼠随机分成正常对照组、模型组、昂丹司琼+地塞米松组、阿瑞吡坦+地塞米松组、姜辣素组及姜辣素+地塞米松组,24 h及72 h记录各组摄食量及食高岭土量。免疫组化方法观察脑和回肠中c-fos蛋白的表达。结果 24 h内,姜辣素组、姜辣素+地塞米松组摄食量增加,啃食高岭土量减少,姜辣素组与姜辣素+地塞米松组相比摄食量增加(P<0.05);48～72h内,姜辣素组食高岭土量减少(P<0.05)。姜辣素组、姜辣素+地塞米松组c-fos阳性表达颗粒的灰度值明显高于模型组(P<0.05)。结论姜辣素可促进摄食并可抑制顺铂所致的大鼠异嗜高岭土行为,地塞米松对姜辣素无辅助作用。Aim Using the model of pica in rats induced by cisplatin,to investigate the antiemetic effect of gingerol on the acute and delayed phases of cisplatin induced nausea and vomiting（CINV）,and to compare the influences of local and central c-fos expression with the help of dexamethasone.Methods The rats were divided into blank control,model control,ondansetron plus dexamethasone group,aprepitant plus dexamethasone group,gingerol and gingerol plus dexamethasone group.Both food and kaolin ingestion were recorded after 24 hours and 72 hours of cisplatin administration.The area postrema of brain and distal ileum tissues were removed when rats were sacrificed.Immunohistochemistry and Image Analysis System were used to compare the differences of c-fos expression inside the cells.Results After 24 hours of cisplatin administration,compared to model control group,the food ingestion of gingerol and gingerol plus dexamethasone groups increased significantly（P0.05）and the kaolin ingestion of those decreased significantly（P0.05）.The food ingestion of gingerol group was more than that of gingerol plus dexamethasone group（P0.05）while the kaolin ingestion had no significant differences（P0.05）.During 48~72 hours of cisplatin administration,compared to the model group,the kaolin ingestion of gingerol and gingerol plus dexamethasone groups decreased significantly（P0.05）,the food ingestion of gingerol group increased significantly（P0.05）,the grey levels of c-fos expression of gingerol and gingerol plus dexamethasone groups were higher significantly.Conclusions Gingerol is able to inhibit cisplatin-induced pica in rats.This kind of ability can not be enhanced by dexamethasone.Gingerol can improve appetites of rats.

关 键 词：姜辣素 异嗜 恶心呕吐 C-FOS 大鼠 地塞米松 

分 类 号：R-332[医药卫生] R284.1