ICSI治疗前原发严重少精症患者Y染色体AZF微缺失分析  

Screening of the microdeletions in the Y chromosome AZF on primary severe oligospermatism before ICSI treatment

作　　者：陆国芳[1] 孙丰涛[1] 任晨春[1] 秦志华[1] 梁玥宏[1] 李树宁[1] 

机构地区：[1]天津医学高等专科学校医学遗传教研室,300222

出　　处：《中国妇幼保健》2012年第11期1673-1675,共3页Maternal and Child Health Care of China

基　　金：天津市教委科研计划项目〔20070211〕

摘　　要：目的:研究胞质内单精子注射(ICSI)治疗前原发严重少精症患者Y染色体无精子症因子(AZF)基因微缺失频率及类型。方法:用多重PCR法,以正常生育男性30例为对照组,对天津70例男性原发严重少精症患者(实验组)进行Y染色体AZF区15个位点微缺失检测。结果:70例严重少精症患者存在缺失9例,总缺失率12.86%;其中AZFb+c缺失6例(8.57%),AZFb+c+d缺失2例(2.86%),AZFb+d缺失1例(1.43%);未见AZFa缺失;对照组各位点均无缺失,2组微缺失频率具统计学差异(P﹤0.05)。结论:男性原发严重少精症患者Y染色体AZF区微缺失频率较高,该检测对ICSI治疗具指导意义。Objective：To study microdeletions rate and type of the Y chromosome azoospermia factor（AZF） in primary severe oligospermatism before intracytoplasmic sperm injection（ICSI） treatment.Methods： Seventy cases of primary severe oligospermatism patients in Tianjin were taken as test group,and thirty cases of normal birth man were taken as control group Fifteen Y-linked sequence tagged sites（STS） were screened by multiple PCR in the two groupsResults：The 9（12.84%）cases of microdeletions were found in the test group.Of the 9 cases,6（8.57%） were microdeletions in AZFb＋c,2（2.86%） were deletions in AZFb＋c＋d,1（1.43%） was deletions in AZFb＋d.Microdeletions in AZFa were no founded.Microdeletions were no founded in the control group There were significant difference between the two groups（P﹤0.05）.Conclusion：There is a high frequency of microdeletion in Y chromosome AZF region in primary severe oligospermatism patients Screening of the microdeletions in the Y chromosome AZF on primary severe oligospermatism is necessary.

关 键 词：胞质内单精子注射 原发严重少精症 Y染色体 AZF微缺失 原发生精障碍 

分 类 号：R394[医药卫生—医学遗传学]