普通念珠藻多糖提取及其体外抑菌活性研究  

Extraction and Antibacterial Activity of Polysaccharides from Nostoc commune

作　　者：钱森和[1] 厉荣玉[2] 魏明[1] 杨超英[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,安徽芜湖241000 [2]皖南医学院,安徽芜湖241001

出　　处：《食品科学》2012年第6期96-99,共4页Food Science

基　　金：安徽省高校省级科学研究项目(KJ2010B285;KJ2011B201)

摘　　要：通过单因素和正交试验对普通念珠藻多糖的提取条件进行优化,并采用滤纸扩散法对普通念珠藻多糖的抑菌作用进行研究。结果表明:提取温度和提取时间对普通念珠藻多糖得率的影响分别达到了极显著和显著水平,最佳提取条件为提取温度90℃、提取时间3h、料液比1:15(g/mL)、提取2次;普通念珠藻多糖对大肠杆菌、枯草芽孢杆菌、粘质沙雷细菌、黑曲霉和假丝酵母具有较好的抑制作用,其对大肠杆菌、枯草芽孢杆菌和粘质沙雷氏菌的最小抑菌质量浓度分别为50、50、25mg/mL,对黑曲霉和假丝酵母的最低抑制质量浓度为100mg/mL。This paper deals with the optimization of process conditions for the extraction of polysaccharides from Nostoc commune using one-factor-at-a-time and orthogonal array design methods.Meanwhile,the antibacterial activity of polysaccharides from Nostoc commune was tested by the filter paper disc diffusion method.The results showed that extraction temperature and time influenced the extraction efficiency of polysaccharides from Nostoc commune at an extremely significant level and at a significant level,respectively.The optimal extraction conditions were temperature of 90 ℃,extraction time of 3 h,material-liquid ratio of 1：15,and repeated extraction number of 2.The resulting extract revealed good antibacterial activity against Escherichia coli,Bacillus subtilis,Serratia marcescens,Aspergillus nigere and Candida albicans with minimum inhibitory concentration（MIC） of respectively 50,50,25,100 mg/mL and 100 mg/mL.

关 键 词：普通念珠藻 多糖 提取 抑菌 

分 类 号：TQ453.2[化学工程—农药化工]