AHSG对胰岛素刺激下的HL-7702细胞中FAS、SREBP-1基因表达的影响  

AHSG impact on expression of FAS and SREBP-1 genes of HL-7702 cells under insulin treatment

作　　者：张春梅[1,2] 陈婷[2] 李平[2] 曹诚[2] 张部昌[1] 

机构地区：[1]安徽大学生命科学学院,合肥230039 [2]军事医学科学院生物工程研究所,北京100850

出　　处：《军事医学》2012年第3期182-185,195,共5页Military Medical Sciences

摘　　要：目的研究纯化后的人胎球蛋白A(AHSG)对HL-7702细胞胰岛素信号通路下游基因FAS、SREBP-1表达的影响。方法通过硫酸铵沉淀、PEG沉淀、DEAE离子交换和鱼精蛋白(protamine)亲和层析方法纯化血浆中的AHSG蛋白;单用胰岛素或胰岛素与AHSG共同处理HL-7702细胞后,提取细胞的RNA并且逆转录成cDNA,通过实时PCR(real-time PCR)方法分析细胞中FAS、SREBP-1基因mRNA的含量。结果 100 nmol/L胰岛素在1、2、3、4、5、6 h都可刺激HL-7702细胞中FAS、SREBP-1的基因表达量增加;在胰岛素刺激HL-7702细胞5 h时,FAS、SREBP-1基因表达量随胰岛素浓度的增加而升高;当HL-7702细胞用20 nmol/L胰岛素与100、300和600μg/ml的AHSG蛋白分别共同处理时,AHSG能抑制FAS、SREBP-1基因的表达,并且在600μg/ml时抑制作用较强。结论胰岛素能刺激HL-7702细胞中FAS、SREBP-1基因表达量的增加,但AHSG蛋白抑制由胰岛素刺激引起的FAS、SREBP-1基因表达量的增加。Objective To study the effect of the purified alpha2-Heremans-Schmid glycoprotein（AHSG） on the expression of insulin signaling pathway downstream genes FAS and SREBP-1 of HL-7702 cells.Methods Using ammonium sulfate precipitation,PEG precipitation,DEAE ion-exchange chromatography and protamine chromatography,a series of steps was conducted for the purification of serum AHSG.Through insulin treatment or insulin and AHSG co-treatment of HL-7702 cells,the RNA of HL-7702 cells was extracted.After reverse transcription and quantitative real-time PCR,the change of the expression of the FAS and SREBP-1 gene was analyzed.Results Insulin of 100 nmol/L stimulated the increase of the FAS and SREBP-1 gene expression of HL-7702 cells in 1,2,3,4,5 and 6 h.When insulin stimulated HL-7702 cells for 5 h,the FAS and SREBP-1 gene expression increased with the insulin concentration.When HL-7702 cells were treated with 20 nmol/L insulin and 100,300 and 600 μg/ml of AHSG,AHSG inhibited the FAS and SREBP-1 gene expression and more strongly at 600 μg/ml.Conclusion Insulin can stimulate the increase of the FAS and SREBP-1 gene expression in HL-7702 cells,but AHSG can inhibit the insulin-stimulation to the increase of the FAS and SREBP-1 gene expression.

关 键 词：AHSG FAS 胆固醇调节元件结合蛋白质1 基因表达 

分 类 号：R363[医药卫生—病理学]