小睑裂患者眼睑和血液中FOXL2表达水平的研究  

Study of the Expression of the Gene FOXL2 in Eyelid and Blood with BPES

作　　者：杨建华[1] 高莎莎[1] 张伟[1] 黄旭东 李艳艳[1] 唐胜建[1] 

机构地区：[1]潍坊医学院整形外科研究所,山东潍坊261042 [2]潍坊市眼科医院

出　　处：《潍坊医学院学报》2012年第1期38-40,F0003,共4页Acta Academiae Medicinae Weifang

基　　金：国家科学自然基金项目（课题编号：30772268）

摘　　要：目的 探讨小睑裂综合征（BPES）患者眼睑和血液中FOXL2 mRNA的相对表达水平与BPES的相关性.方法 收集小睑裂综合征患者（A组）和正常人（B组）的眼睑组织和外周静脉血液.TRIzol提取总RNA,反转录为cDNA.应用荧光定量PCR技术检测FOXL2的相对表达量,方差分析和t检验分析数据差异,Livak（2-△△Ct）法分析表达差异.结果 FOXL2 mRNA的表达水平在A,B两组间的差异均具有统计学意义,P〈0.05.无论是在眼睑还是在血液中A组FOXL2的△Ct值大于B组,可以认为FOXL2的表达水平为 A组低于B组,且两者具有一致性.结论 FOXL2与BPES具有一定相关性,FOXL2在眼睑和血液中的低表达可能与BPES的发病相关.Objective To deteet the expression of FOXL2 in eyelid and blood with BPES and define the relation of FOXL2 to BPES disease and further study the possible pathngenesis. Methods The eyelid and peripheral venous blood with BPES（Group A） anti normal（Group B） was collected. RNA was extraeted by TRlzol and then was reverse transcripled to eDNA. RT-Q-PCR was applied to detect the relative mRNA expression levels of FOXI2 in eyelid and blood from Group A and Group B. ANOVA and two paired sample t test and Livak（2-△△Ct） method were used to analyze the differences between two data sets. Results Whether in the eyelid or blood,the difference of ACt value between the two groups were statistically significant. In eyelid （ P 〈 0.05 ）, and A Ct of Group A （ 1. 9933 ± 1. 2386 ） was higher than Group B （ 1. 1647 ±0. 6593 ）. In blond （ P 〈 0.05 ）, and ACt of Group A （4. 5260 ± 1. 9266 ） was higher than Group B（3. 7420 ±1. 9266）. And the difference in A and B are consistent. This indieated that FOXL2 expression in Group A was lower than in Group B in both eyelid anti blood. Conclusion FOX12 is associated with BPES,low expressinn of FOXI2 in eyelid and blood might be relaled to BPES pathogenesis.

关 键 词：小睑裂综合征 FOXL2 荧光定量PCR 

分 类 号：R777.1[医药卫生—眼科]