一种从马鹿粪便中提取DNA的改进方法  

An Improved Method for DNA Extraction from the Faeces of Cervus elaphus

作　　者：日沙来提·吐尔地[1] 艾斯卡尔·买买提[1] 日孜汗·阿布地艾尼[1] 阿米拉·阿布来提[1] 马合木提·哈力克[1] 

机构地区：[1]新疆大学生命科学与技术学院资源生物研究所,新疆乌鲁木齐830046

出　　处：《安徽农业科学》2012年第10期5739-5740,5754,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(31060152);新疆维吾尔自治区自然科学基金(2010211A02);新疆大学自治区重点学科-动物学科资助

摘　　要：[目的]介绍一种从马鹿粪便中提取DNA的改进方法。[方法]在传统的CTAB裂解法的基础下,根据马鹿粪便的特征进行改进所得的DNA提取方法。[结果]采用该方法从天山马鹿粪便中提取了高质量的粪便DNA并扩增出了天山马鹿线粒体细胞色素b基因片段,通过测序检测,同时以提取的马鹿肌肉和皮毛样品DNA作为对照,进一步证实了提取的可靠性。[结论]该方法提取过程中无需使用蛋白酶K;所提取的DNA无需使用DNA纯化试剂盒纯化,可直接用于PCR扩增,因此,试验费用很低。[Objective] To introduce an improved method for DNA extraction from the faeces of red deer.[Method] Based on the traditional method of CTAB lysis,we proposed an improved DNA extraction method according to the characteristics of red deer feces.[Result] The improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene.The sequencing detection,using the muscle and skin DNA of red deer ad the control,further confirmed the reliability of the method.[Conclusion] The method requires no proteinase K in the process of extraction,and the extracted DNA can be used for PCR amplification directly without the purification of DNA purification kit,thus,the cost for the test is very low.

关 键 词：马鹿 非损伤性取样 改进的DNA提取方法 粪便DNA 

分 类 号：S811.3[农业科学—畜牧学]