高效液相色谱法同时测定食品中6种对羟基苯甲酸酯  

Simultaneous Determination of Six Kinds of 4-Hydrobenzoic Acid Ester in Food Using High Performance Liquid Chromatography-Diode Array Detection

作　　者：曹淑瑞[1,2] 刘治勇[3] 张雷[1] 李贤良[1] 王国民[1] 唐柏彬[1] 袁若[2] 

机构地区：[1]重庆出入境检验检疫局,重庆400020 [2]西南大学化学化工学院,重庆400715 [3]重庆医科大学药学院,重庆400016

出　　处：《分析化学》2012年第4期529-533,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20675064);重庆市自然科学基金(No.CSTC-2009BA1003);西南大学高新技术培育基金(No.XSGX02)项目资助

摘　　要：建立了同时检测食品中6种对羟基苯甲酸酯的高效液相色谱-二极管阵列检测器(HPLC-DAD)定量分析方法。样品用乙腈提取,经LC-C18固相萃取柱净化,采用Agilent C18色谱柱分离,甲醇/水溶液梯度洗脱,二极管阵列检测器检测,外标法定量。结果表明:6种对羟基苯甲酸酯在1.0～500.0mg/L范围内线性关系良好(r≥0.9997);检出限为0.0016～0.0081mg/L(S/N=3);6种对羟基苯甲酸酯各添加水平在经表面处理的新鲜蔬菜、水果、醋、碳酸饮料及牛奶,食品馅料中的回收率为85.1%～95.4%;相对标准偏差为3.6%～10.9%。A method was developed for the determination of preservatives of six 4-hydrobenzoic acid ester by HPLC-DAD.The samples of 4-hydrobenzoic acid ester were extracted by acetonitrile and purified by LC-C18 SPE.The analytes were separated on an Agilent C18 column,eluted by gradient with methanol and water,detected by diode array detector,and quantified by external standard method.The calibration curve was linear over the concentration range of 1.0-500.0 mg/L（r≥0.9997）.The lower limit of quantification in food was 0.0016-0.0081 mg/L.The relative recoveries of six kinds of 4-hydrobenzoic acid ester in vegetables and fruit of surface treatment,vinegar,carbonated beverages,milk,and food fillings were 85.1%-95.4%,and relative standard deviations were 3.6%-13.0%.

关 键 词：高效液相色谱法 对羟基苯甲酸酯 固相萃取 食品 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.72[轻工技术与工程—食品科学与工程]