正交设计优选檀香叶总黄酮提取工艺  

Optimization of the extraction technology of total flavonoids from leaves of Santalum album L.by orthogonal experiment

作　　者：余文新[1] 庄满贤[2] 林励[2] 邓瑞云[2] 彭丽诗[2] 李丽月[2] 

机构地区：[1]广东省中医院药学部,广东广州510120 [2]广州中医药大学,广东广州510006

出　　处：《食品工业科技》2012年第8期284-286,共3页Science and Technology of Food Industry

基　　金：高等学校博士学科点专项科研基金(20094425110009);国家工信部中药材扶持项目(2009060)

摘　　要：以檀香叶为原料,在单因素实验基础上,以乙醇浓度、提取时间、料液比、提取次数为考察因素,以总黄酮含量为考察指标,采用正交实验优化最佳提取工艺。在实验影响因素中,影响程度从大到小依次为乙醇浓度>提取次数>料液比>提取时间。实验结果表明:檀香叶总黄酮的最佳提取工艺为乙醇浓度60%,提取时间20min,料液比1:40,提取次数为1次。此工艺条件较为稳定,檀香叶总黄酮可达3.24%。The extraction technology of total flavonoids from Leaves of Santalum album L. was optimized by orthogonal experiment with the content of total flavonoids as index and with ethanol concentration,extracting time, the ratio of raw materials to solvent and extracting frequency as factors.The influences of factors to different degree with an order of ethanol concentration, extracting frequency, the ratio of raw materials to solvent, extracting time. The optimal extraction technology was as follow, the concentration of ethanol was 60%, the extracting time was 20min,the ratio of raw materials to solvent was 1：40 and extracting frequency was 1 time.The extraction technology was steady for the extraction of total flavonoids from Leaves of Santalum album L., and the content of otal fiavonoids from Leaves of Santalum album L .was 3.24%

关 键 词：檀香叶 总黄酮 正交设计 提取工艺 

分 类 号：TS201.2[轻工技术与工程—食品科学]