响应面优化龙眼核原花青素的醇提工艺  

Optimization of ethanol extraction of proanthocyanidins from longan seed using response surface analysis

作　　者：彭晓莉[1] 刘新[1] 陈卫中[1] 程道梅[1] 冷言冰[1] 

机构地区：[1]成都医学院公共卫生系,四川成都610083

出　　处：《食品工业科技》2012年第8期305-308,313,共5页Science and Technology of Food Industry

摘　　要：目的:优化龙眼核原花青素醇提条件。方法:在单因素实验的基础上,应用Box-Behnkenk中心组合实验和响应面分析法对龙眼核原花青素的醇提条件进行优化。结果:通过单因素实验确定乙醇体积分数、料液比和提取温度为优化对象。建立二次多项式回归方程预测模型Y=1.15+0.034A+0.094B+0.004C-0.036AB+0.044AC-0.11BC-0.24A2-0.23B2-0.087C2。响应面分析确定适合的反应条件为:乙醇体积分数40%,料液比1:23(mg/mL),提取温度为39℃。预测响应值为1.16%,验证龙眼核原花青素得率为1.12%。结论:响应面分析法确定的提取条件合理,验证值与预测值接近,可以用于龙眼核原花青素的提取。Objective： To optimize ethanol extraction of proanthocyanidins from Iongan seed. Methods： A BoxBehnken design in response surface analysis was used to optimization of ethanol extraction of proanthocyanidins from Iongan seed.Results-Ethanol concentration, ratio of raw material to solvent （W/V）and temperature were selected as experimental factors.The optional conditions of ethanol extraction technology were as follows：ethanol concentration was 40%, ratio of raw material to solvent was 1：23, temperature was 39℃.The mathematical model was Y = 1.15 ＋ 0.034A ＋ 0.094B ＋ 0.004C-0.036AB ＋ 0.044AC-0.11BC-0.24A^2-0.23 B^2-0.087C^2.The predicted values was 1.16%.The measured value of proanthocyanidins yield from Iongan seed was 1.12%.Conclusion ：The obtained model was credible and could be used for actual prediction.

关 键 词：龙眼核 原花青素 响应面 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]