黄酒中生物胺的测定  

The Determination of Biogenic Amine in Yellow Rice Wine

作　　者：玉澜[1] 谢济运[1] 蓝峻峰[1] 

机构地区：[1]柳州师范高等专科学校,广西柳州545004

出　　处：《安徽农业科学》2012年第11期6498-6500,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为测定黄酒中生物胺的含量寻求一种快速、简单、灵敏的检测方法。[方法]以丹磺酰氯为衍生剂,室温离子液体作为萃取、富集、衍生介质来测定黄酒中生物胺的含量。[结果]用室温离子液体[HMIM]PF6为介质来衍生、萃取和富集黄酒中的生物胺。通过试验得出比较理想的衍生条件:衍生剂量1.5 ml,硼砂缓冲液的pH 9.10,硼砂缓冲液量为2.0 ml,1.0 ml[HMIM]PF6离子液,超声时间为30 min。所得出的6种生物胺回收率为87.5%～109.8%,相对标准偏差为0.75%～3.21%,检出限量为0.005～0.025μg/ml。该方法将衍生、萃取和富集3个步骤集于一体,大大简化了试验,离子液体可有效促进分离,稳定性良好,所建立的方法线性范围良好。[结论]以丹磺酰氯为衍生剂,室温离子液体作为萃取、富集、衍生介质来测定生物胺的含量,是测定黄酒及其他饮料中痕量生物胺含量的一种快速、简单、灵敏的检测方法。[Objective] The aim was to seek a rapid,simple and sensitive detection method for determining the content of biogenic amine in yellow rice wine.[Methods] Taking dansyl chloride as derivating agent and using room temperature ionic liquids（RTILs） as extractive,enrichment and derivative media,to determine the content of biogenic amine in yellow rice wine.[Result] Using RTILs PF6 as media to derive,extract and enrich biogenic amine in yellow rice wine.Based on the test,the ideal derive conditions were obtained： derivative dose 1.5 ml,borax buffer solution pH 9.10,borax buffer solution 2.0 ml,PF6 ion liquid 1.0 ml,supersonic time 30 min.The recovery rate of 6 biogenic amines was among 87.5%-109.8%,relative standard deviation was among 0.75%-3.21%,the detection limit was among 0.005-0.025 μg/ml.The method was the integration of extractive,enrichment and derivative,it simplified the test greatly,ionic liquid could promote the isolation effectively,the stability was better and the linear range of the established method was better.[Conclusion] The method is a rapid,simple and sensitive detection method for determining the content of trace biogenic amine in yellow rice wine and other beverages.

关 键 词：室温离子液 生物胺 高效液相色谱 

分 类 号：S18[农业科学—农业基础科学] O622.6[理学—有机化学]